Registration Dossier
Registration Dossier
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EC number: 201-557-4 | CAS number: 84-74-2
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
Description of key information
Hulzebos et al., 1993 studied the toxicity of 76 priority pollutants to lettuce (Lactuca sativa) in soil and in nutrient solution. In the first case a static and in the latter a semistatic exposure was established. The EC 50 of DBP after 7 days was 387 µg/g soil dw with 95% confidence limits: 262 -570 µg/g soil dw (nominal test concentration).
Key value for chemical safety assessment
- Short-term EC50 or LC50 for terrestrial plants:
- 387 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 200 mg/kg soil dw
Additional information
Ma et al., 2013 evaluated the individual toxicity of di-n-butyl phthalate (DnBP) and bis (2-ethylhexyl) phthalate (DEHP) to sown rape (Brassica chinensis L.) seeds within 72 h (as germination stage) and seedlings after germination for 14 days by monitoring responses and trends of different biological parameters.
No significant effects of six concentrations of DBP ranging from 0 (not treated/NT) to 500 mg/kg on germination rate in soil were observed. However, root length, shoot length, and biomass (fresh weight) were inhibited by DBP. Among all the estimated growth parameters, root elongation may be considered the most sensitive physical parameter with the lowest IC50 under exposure to DnBP, with a value of 456.4 mg/kg of soil.
According to EU RAR for DBP, 2004 in a limited greenhouse experiment in which seeds of corn Zea mays were planted in a sandy soil containing 0 to 20,000 mg DBP/kg, germination was not affected at any concentration. After 3 weeks of exposure, plant height and shoot fresh weight were reduced significantly at 2,000 mg/kg (17% and 25%, respectively); a concentration of 200 mg/kg was without effect (NOEC). After planting a second group of seeds in the soils, plant growth was only reduced at 20,000 mg/kg,while concentrations <2,000 mg/kg were without effect. These results indicate that plant available DBP levels have decreased through complex formation with soil components and/or by degradation (Shea et al., 1982).
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