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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

A study was conducted according to DIN 38412 Teil 2. 
A publication by McFarlan and Norris (1958) is available, investigating the effect of 2-amino-2-(hydroxymethyl)-1,3-propanediol on fish, but the test method does not comply with today’s standards for the assessment of toxicity.

Key value for chemical safety assessment

Additional information

In the publication by McFarlan and Norris (1958), 29 species of fishes (in total 59 fishes), were exposed to Tris-buffer in order to determine if harmful properties existed. All but four species were marine forms. Marine fish were exposed for 12.5 h or 48 h, depending on test species. Following exposure, they were transferred to untreated water and observed for 30 days. Freshwater fish were exposed for nine days. One death occurred during the experiment, but it was probably due to starvation, since the fish were not fed until the fourth day.


Therefore, the assessment of fish toxicity was based on a study conducted according to (DIN 38412 Teil 2)conducted with a 40% concentration of trometamol by Woermann and Lamberty (1990).  The test organism Leuciscus idus melanotus (golden orfe) was exposed to trometamol

in a static system for 96 hours, at the test concentrations of 10 g/L. No effects were observed at 10 g/L of product containing 40% of the substance, or 4g/L of Tris amino.