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EC number: 215-693-7 | CAS number: 1344-37-2 This substance is identified in the Colour Index by Colour Index Constitution Number, C.I. 77603.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Long-term toxicity to fish
Administrative data
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Scientifically acceptable publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Chronic toxicity of water-borne and dietary lead to rainbow trout (Salmo gairdneri) in lake ontario water
- Author:
- Hodson PV, Blunt BR, Spry DJ
- Year:
- 1 978
- Bibliographic source:
- Water Research Vol. 12: 869 - 878
Materials and methods
- Principles of method if other than guideline:
- Acute lethal and chronic sublethal toxicity was tested in continous-flow bioassays. (Method according to Sprague J. B. (1969) Measurement of pollutant toxicity to fish. I. Bioassay methods for acute toxicity. Water Res. 3, 793-821.)
Three different experiments were conducted - GLP compliance:
- no
Test material
- Reference substance name:
- lead nitrate
- IUPAC Name:
- lead nitrate
- Details on test material:
- - Name of test material (as cited in study report): lead nitrate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Erin Conservation Holdings LTD, Erin, USA
- Weight at study initiation (mean and range, SD): 6.5 g (experiments I, II); 10.5 (Experiment III)
- Feeding during test
- Food type: EWOS, Astra Chemicals Ltd. (except feeding experiment); 2.4 g lead nitrate dissolved in 10 ml was added to homogenised beef liver. The powdered liver was added to commercial fish food.
- Amount: ad libitum (except feeding experiment)
- Frequency: daily
- Health/mortality:
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 wk
Test conditions
- Hardness:
- 135 +/- 2 mg/l
- Test temperature:
- 10.3 - 11.2 °C
- pH:
- 7.7 +/- 1.8
- Dissolved oxygen:
- 6.1 - 12 mg/l
- Nominal and measured concentrations:
- Nominal test concentrations:
Experiment I: control, 0.6 - 8 mg/l
Experiment II: control, 10, 18, 32, 56, 100 µg/l
Experiment III: control 100, 180, 320, 560, 10000 µg/kg - Details on test conditions:
- TEST SYSTEM
- Test vessel: polyethylene tank 30 x 30x 30 cm
- Fill volume: 20 l
- Renewal rate of test solution (frequency/flow rate): flow for each tank 300 ml/min (experiment I, II)/ 400 ml/min (experiment III)
- No. of organisms per vessel: 20 (I), 23 (II)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
:
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: municipal water from lake Ontario. Filtrated (sand, charcoal). Alum was flocculated, chlorine supplemented.
- Alkalinity: 90 +/- 4 mg/l
- Conductivity: 245 +/- 9 µmhos/cm
OTHER TEST CONDITIONS
- Photoperiod: 16:8 h day-night regime
Results and discussion
- Details on results:
- 25 and 7 % mortality respectively in chronic and food experiments due to aggression.
Any other information on results incl. tables
Lead concentrations in body tissue of fish increased linearly with the lead concentration in water. The highest lead concentrations were observed in opercular bone followed by gills and kidney. Starting at a lead concentration of 13 µg/l lead accumulation in fish tissues occurred throughout all tested concentrations. Whereas the lead concentrations in the tissues did not increase when the lead concentrations in food were enhanced.
Hematological effects were detected in fish exposed to lead via water of at least 13 µg lead/l. The authors conclude from their data that lead induced an accelerated mortality and removal of mature red cells and an acceleration of hemopoiesis. Furthermore they suggest that a reduction of cellular hemoglobin occurred. At a concentration of 120 µg/l some test fish had black tails.
Applicant's summary and conclusion
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