Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-532-0 | CAS number: 96-76-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: inherent biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD 302 C)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 302 C (Inherent Biodegradability: Modified MITI Test (II))
- Deviations:
- no
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Batch Number 2,4-AP/307
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- other: a mixed population of activated sewage sludge microorganisms
- Details on inoculum:
- - Source of inoculum: Activated sewage was prepared by sampling 10 different sites around the UK in accordance with OECD Guideline No. 302C
- Preparation: the mixed sludge was fed daily with 0.1% synthetic sewage and maintained on a constant aeration at 25 ± 1C
- Usage rate: equivalent to 100 mg dry weight/l - Duration of test (contact time):
- 28 d
- Initial conc.:
- 30 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- - Test temperature: 25 ± 1C
- Aeration of dilution water: constant
- Continuous darkness: yes
- Agitation: by magnetic stirrers
- Criteria: oxygen consumption measured by direct manometer reading
- Observations: manometer volumes were recorded daily - Reference substance:
- aniline
- Test performance:
- TOC analysis of phenol, 2,4-bis (1,1 -dimethylethyl) was not possible due to the insoluble nature of the test material.
A modified Miti test was carried out due to the insoluble nature of the test material.
A COD value of 2.85 mg O2/mg was obtained for the test material.
For % degradation calculation a ThOD of 2.95 mg O2/mg was used based on the following approximate values:
Molecular formula: C14H22O
Molecular weight: 206 - Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- % Biodegradation
0% 0-28 days
All % Biodegradation values have been corrected for blank values - Parameter:
- COD
- Value:
- 2.85 other: mg O2/mg test material
- Results with reference substance:
- Aniline attained 74% biodegradation after 14 days thereby confirming the suitability of the inoculum and culture conditions. Aniline attained 4% degradation within 7 days. However this is not considered to affect the overall integrity of the study given that aniline attained 74% degradation within 14 days. Total organic carbon analysis of the test media at day 0 and day 28 showed that aniline attained 87% degradation within 28 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not inherently biodegradable
- Conclusions:
- Phenol, 2,4-bis (1,1-dimethylethyl)- attained negligible degradation within 28 days calculated from oxygen uptake (% of COD and ThOD) and , therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines
- Executive summary:
The inherent biodegradability of the test substance phenol 2,4 -bis(1,1 -dimethylethyl) was evaluated. The method used followed that described in the OECD Guidelines for Testing of Chemicals / Section 2: Degradation and Accumulation, Test No. 302C "Inherent Biodegradability, Modified MITI Test (II). A mixed population of activated sewage sludge microorganisms was used as inoculum and aniline as reference substance. Initial concentration of test substance was 30 mg/l and reference substance 100 mg/l. Phenol 2,4 -bis(1,1-dimethylethyl) attained negligible degradation within 28 days calculated from oxygen uptake (% of COD and ThOD) and so, therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines. TOC analysis of phenol 2,4 -bis(1,1-dimethylethyl) was not possible due to the insoluble nature of the test material. A modified Miti test was carried out due to this fact. A COD value of 2.85 mg O2/mg was obtained for the test material.
Reference
Day |
% Biodegradation |
|
Phenol 2,4-bis (1,1-dimethylethyl) |
Aniline |
|
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 |
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 |
0 0 0 0 1 2 4 12 17 18 41 64 73 74 73 74 81 86 86 80 90 89 87 86 83 81 81 88 |
Description of key information
Negligible degradation within 28 days (% of COD and ThOD), cannot be considered as inherently biodegradable (OECD 302 C).
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Two studies have been conducted which assess biodegradability in water (activated sludge respiration inhibition test).
1. Inherent biodegradability, Modified MITI Test (II). (OECD 302C)
2. BOD-Test for Insoluble Substances
In the first test, the inherent biodegradability of 2,4-DTBP was evaluated. The method used followed that described in the OECD Guidelines for Testing of Chemicals / Section 2: Degradation and Accumulation, Test No. 302C "Inherent Biodegradability, Modified MITI Test (II). A mixed population of activated sewage sludge microorganisms was used as inoculum and aniline as reference substance. Initial concentration of test substance was 30 mg/l and reference substance 100 mg/l. 2,4-DTBP attained negligible degradation within 28 days calculated from oxygen uptake (% of COD and ThOD) and therefore, cannot be considered as inherently biodegradable under the strict terms and conditions of the OECD Guidelines. TOC analysis of 2,4-DTBP was not possible due to the insoluble nature of the test material. A modified MITI test was carried out due to this fact. A COD value of 2.85 mg O2/mg was obtained for the test material.
In the second test, 2,4-DTBP was evaluated for its biodegradability according to ISO Draft (BOD Test for Insoluble Substances). Test substance, 6.3, 7.6 and 6.8 mg, 2,4-DTBP/flask and reference substance, diethylene glycol (13.8, 13.8 and 13.7 mg/flask) were weighed into flasks and samples were taken weekly over 28 days to measure the oxygen content.
The test material attained negligible degradation (2%) within 28 days calculated from oxygen uptake (% of ThOD) and therefore, cannot be considered as biodegradable.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
