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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Rationale for reliability A published study but one which contains sufficient experimental detail to be able to judge it reliable for risk assessment purposes.

Data source

Reference Type:
Metabolic studies with diethylene glycol monobutyl ether acetate (DGBA) in the rat
Deisinger PJ, Guest D
Bibliographic source:
Xenobiotica, 19, 9, 981-9

Materials and methods

Objective of study:
Principles of method if other than guideline:
To establish the rate of in vitro enzymic hydrolysis in the blood of rats of the substance to the parent glycol ether 2-(2-butoxyethoxy)ethanol.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-(2-butoxyethoxy)ethyl acetate
EC Number:
EC Name:
2-(2-butoxyethoxy)ethyl acetate
Cas Number:
Molecular formula:
2-(2-butoxyethoxy)ethyl acetate
Details on test material:
- Name of test material (as cited in study report): Diethylene glycol monobutyl ether acetate (DGBA)
- Physical state: liquid
- Analytical purity: 95%
- Supplier: Kodak Laboratory CHemicals (ROchester, NY)
- Impurities (identity and concentrations): 2-(2-ethoxyethoxy)ethyl acetate, diethyl phthalate, methyl substituted DGBA.
- Radiochemical purity (if radiolabelling): 97.1%
- Specific activity (if radiolabelling): 0.951uCi/mole
- Locations of the label (if radiolabelling): no data
- Supplier: Wizard Laboratories (Davis, CA)

Test animals

Details on test animals or test system and environmental conditions:
- Source: Charles River Laboratories, Wilmington, MA
- Weight at study initiation: 200-250g
- Individual metabolism cages: yes/no
- Diet: Purina Chow 5002, ad libitum
- Water: ad libitum

Results and discussion

Toxicokinetic / pharmacokinetic studies

Toxicokinetic parameters
Toxicokinetic parameters:
half-life 1st: 3 minutes

Any other information on results incl. tables

Metabolism of the substance in blood through hydrolysis to 2 -(2 -butoxyethoxy)ethanol (22BEE) was rapid. By 12 minutes the concentration of the 2 -(2 -butoxyethoxy)ethyl acetate was reduced by a mean of 94% to 0.2mM where it reached a plateau. Similarly the concentration of the metabolite 22BEE rose rapidly to a plateau of 4.1mM. No hydrolysis was seen in saline solution and no significant decrease in 22BEE concentration was seen when this was incubated in either blood or saline.

Applicant's summary and conclusion

The hydrolysis of 2-(2-butoxyethoxy)ethyl acetate to the parent glycol ether 2-(2-butoxyethoxy)ethanol in blood is very rapid. This suggests that
data for the latter is likely to be a very good surrogate for the systemic toxicity of the former.
Executive summary:

In an in vitro study to establish the rate of in vitro metabolism of 2 -(2 -butoxyethoxy)ethyl acetate in the blood of rats to the parent glycol ether 2-(2-butoxyethoxy)ethanol, hydrolysis was found to be very rapid with a half life of approximately 3 minutes. This suggests that data for the latter is likely to be a very good surrogate for the systemic toxicity of the former.