1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1994-02-17 to 1994-05-26

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

other: OECD guideline 208 "Terrestrial Plants, Growth Test", adopted 4 April 1984

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil:
Main test 1: 0,16 g of test substance were mixed with 20 g silica sand and 1680 g soil
Main test 2: 0,06 g of test substance were mixed with 20 g silica sand and 580 g soil

- Controls: yes, soil

Species:

Triticum aestivum

Plant group:

Monocotyledonae (monocots)

Details on test organisms:

- Common name: wheat
- Plant family: Poaceae
- Variety: not mentioned
- Source of seed: Hüls Gartenbaubetrieb, Marl, Germany
- Prior seed treatment/sterilization: not mentioned
- Historical germination of seed (germination of seed lot tested): not tested
- Seed storage: not mentioned

Species:

Brassica alba

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: mustard
- Plant family: Brassicaceae
- Variety: not mentioned
- Source of seed: Hüls Gartenbetriebe, Marl, Germany
- Prior seed treatment/sterilization: not mentioned
- Historical germination of seed (germination of seed lot tested): not tested
- Seed storage: not mentioned

Species:

Lepidum sativum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: garden cress
- Plant family: Brassicaceae
- Variety: not mentioned
- Source of seed: Hüls Gartenbetriebe, Marl, Germany
- Prior seed treatment/sterilization: not mentioned
- Historical germination of seed (germination of seed lot tested): not tested
- Seed storage: not mentioned

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

yes

Total exposure duration:

17 d

Remarks:

18 days for Lepidum sativum and Triticum aestivum

Post exposure observation period:

none

Test temperature:

Main test 1: 22 - 25 °C
Main test 2: 22 - 26 °C

pH:

Main test 1: 6.7
Main test 2: 6.8

Moisture:

approx. 60 % (maximum holding water capacity) at the beginning of the test, no further details mentioned

Details on test conditions:

TEST SYSTEM
- Testing facility: partly air-conditioned room, no further details mentioned
- Test container (type, material, size): flowerpots of black plastics (diameter top/bottom approx. 8/6 cm; height approx. 6 cm)
- Amount of soil: 100 g test substrate per planting pot
- Method of seeding: not mentioned
- No. of seeds per container: 5
- No. of plants (retained after thinning): no thinning performed
- No. of replicates per treatment group: 20 (for each plant species 4 replicates with 5 seeds each)
- No. of replicates per control: 20 (4 replicates with 5 seeds each)



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): corresponded to the requirements of the guideline, no further details mentioned
- Organic carbon (%): < 1.5 %
- Maximum water holding capacity (in % dry weigth): approx. 60 %
- CEC: not mentioned
- Pretreatment of soil: sieved with 0.5 cm mesh, no further details mentioned
- Storage (condition, duration): not mentioned


NUTRIENT MEDIUM: not used


GROWTH CONDITIONS
- Photoperiod: 16 h light, 8 h darkness
- Light source: not mentioned
- Light intensity and quality: 1000 - 2000 Lux (day) in main test 1 and approx. 1500 Lux (day) in main test 2, approx. 50 Lux (night)
- Day/night temperatures: 22 - 26 °C, not differentiated between day and night
- Relative humidity (%): 90 - 98 %
- Wind velocity: not applicable
- Air exchange rate: not mentioned
- Watering regime and schedules:
- Water source/type: not mentioned
- Volume applied: not mentioned
- Interval of applications: once to twice per week
- Method of application: not mentioned
- Any pest control method/fertilization (if used): not used

ACCLIMATION PERIOD: not mentioned


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : emergence of seeds, weight of overground part of plant at the end of the test


- Phytotoxicity rating system (if used): not used

Nominal and measured concentrations:

100 mg /kg soil (nominal)

Reference substance (positive control):

no

Species:

Triticum aestivum

Duration:

18 d

Dose descriptor:

LC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

seedling emergence

Species:

Triticum aestivum

Duration:

18 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Species:

Brassica alba

Duration:

17 d

Dose descriptor:

LC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

seedling emergence

Species:

Brassica alba

Duration:

17 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Species:

Lepidum sativum

Duration:

18 d

Dose descriptor:

LC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

seedling emergence

Species:

Lepidum sativum

Duration:

18 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth

Details on results:

SEEDLING EMERGENCE
- Percent seedling emergence: see below
- Percent survival: see below
- Plant height: not measured
- Dry weight: not measured, fresh weight was measured: see below
- Abnormal seed development or appearance: not determined
- Other effects: none

Results with reference substance (positive control):

no reference substance tested

Reported statistics and error estimates:

no statistics performed as the mean values of the 100 mg/kg group were equal or slightly better than the values of the control group

- Nominal concentrations: 100 mg/kg dry soil

- Effect data: Wheat

- Emergence: 100% (20/20)

- mean fresh weight: 336 ± 90 mg

Control:

- Emergence: 85% (17/20)

- mean fresh weight: 325 ± 73 mg

- Effect data: Cress

- Emergence: 100% (20/20)

- mean fresh weight: 90 ± 24 mg

Control:

- Emergence: 90% (18/20)

- mean fresh weight: 89 ± 20 mg

- Effect data: Mustard

- Emergence: 90% (18/20)

- mean fresh weight: 172 ± 32 mg

Control:

- Emergence: 90% (18/20)

- mean fresh weight: 160 ± 42 mg

Validity criteria fulfilled:

yes

Remarks:

At least 85% of the control seeds produced healthy seedlings.

Conclusions:

All tested species showed no phytotoxic effects at the tested concentration of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters compared to the control. The LC50 for seedling emergence and the EC50 for growth of Triticum aestivum, Lepidium sativum and Brassica alba were therefore observed to be greater than 100 mg/kg dry soil.

Executive summary:

The test item 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters was tested on effects of seedling emergence and seedling growth on three non-target plant species representing three plant families according to OECD Guideline for Testing of Chemicals No. 208.

For the test the following plant species were selected: wheat (Triticum aestivum) for Category 1, mustard (Brassica alba) for Category 2 and cress (Lepidium sativum) for Category 3. A control and one limit concentration of 100 mg/kg dry soil (nominal) were tested for each species. For the limit concentration the test substance was mixed with silica sand and then incorporated into soil in which the seeds were sown. The number of seedlings that emerged were recorded. After 17 days in the test with Brassica alba resp. 18 days in the test with Triticum aestivum and Lepidium sativum the plants were harvested and weighed.

All tested species showed no phytotoxic effects at the tested concentration of WITAMOL 218 compared to the control. The LC50 for seedling emergence and the EC50 for growth of Triticum aestivum, Lepidium sativum and Brassica alba were therefore observed to be greater than 100 mg/kg dry soil.

Description of key information

All tested species showed no phytotoxic effects at the tested concentration of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters compared to the control. The LC50 for seedling emergence and the EC50 for growth of Triticum aestivum, Lepidium sativum and Brassica alba were therefore observed to be greater than 100 mg/kg dry soil.

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:

100 mg/kg soil dw