1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1994-07-28 to 1994-08-11

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)

Principles of method if other than guideline:

Toxizitaetstest fuer Regenwuermer nach 88/302 EWG

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Method of mixing into soil: The test substance was mixed with silica sand and afterwards added to soil substrate in a plastic bag. Water was added to a theoretical water content of approx. 40% and the substrate was thoroughly mixed

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH&Co. Produktions- und Vertrieb KG, Kapellenstraße 25, Wiesbaden, Germany
- Age at test initiation: at least 2 months
- Weight at test initiation: 301 - 599 mg (mean weight control group: 410 mg; mean weight test substance group: 392 mg)

ACCLIMATION
- Acclimation period: not mentioned

Study type:

laboratory study

Substrate type:

other: mixture of peat and horse dung (1:1)

Limit test:

yes

Total exposure duration:

14 d

Post exposure observation period:

no

Test temperature:

20 ± 2 °C

pH:

5.6 - 5.7 at the beginning of the test

Moisture:

40% relative to weight of dry substrate

Details on test conditions:

TEST SYSTEM
- Test container (material, size): Makrolon cages with perforated plexiglass covers, size not mentioned
- Amount of soil or substrate: not mentioned
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: not applicable

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): mixture of peat and horse dung (1:1)
- Organic carbon (%): not mentioned
- Maximum water holding capacity (in % dry weigth): approx. 40 %
- CEC: not mentioned
- Pretreatment of soil: not mentioned
- Storage (condition, duration): fresh prepared
- Stability and homogeneity of test material in the medium: not mentioned

OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity: approx. 400-600 lux

VEHICLE CONTROL PERFORMED: not applicable

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Range finding study: not performed

Nominal and measured concentrations:

nominal concentration: 1000 mg/kg dry substrate

Reference substance (positive control):

yes

Remarks:

chloracetamide, historical data

Duration:

14 d

Dose descriptor:

LC10

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

- Mortality at end of exposure period: none
- Total mass of adults at beginning of test: total weight in control 16.4 g; total weight in test substance: 15.7 g

Results with reference substance (positive control):

RESULTS: TEST WITH REFERENCE SUBSTANCE (historical data, carried out from 1994-03-03 to 1994-03-17)
- Concentrations: 0, 10, 30 and 100 mg 2-chloroacetamide/kg dry soil
- Results (mortality):
0 mg/kg: 1/20 (5%) after 7 and 14 days, respectively;
10 mg/kg: 0/20 (0%) after 7 and 14 days, respectively;
30 mg/kg: 18/20 (90%) after 7 and 14 days, respectively;
100 mg/kg: 17/20 (85%) after 7 and 14 days, respectively.

Reported statistics and error estimates:

As no mortality was recorded with the test substance, no statistics were performed.

RESULTS: EXPOSED

- Nominal concentrations: 1000 mg/kg dry soil

- Effect data (Mortality): 0/40 (0%) after 7 days; 0/40 (0%) after 14 days

RESULTS: CONTROL

- Effect data (Mortality): 0/40 (0%) after 7 and 14 days

Validity criteria fulfilled:

yes

Conclusions:

Not toxic under test conditions at a limit concentration of 1000 mg/kg.

Executive summary:

The acute toxicity of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters to the earthworm eisenia foetida was determined during a 14 day exposure period according to EEC Methods for Determination of Ecotoxicity, Directive 88/302/EEC. For the limit test with a concentration of 1000 mg/kg dry soil, the test substance was mixed with silica sand and afterwards added to soil substrate. After 7 and 14 days the effects of the test substance on the earthworms' survival were examined.

No mortality occurred at the tested concentration after 7 and 14 days. The 14 day LC50 of WITAMOL 218 to Eisenia foetida was observed to be > 1000 mg/kg dry soil.

Description of key information

The 14 day LC50 of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters to Eisenia foetida was observed to be > 1000 mg/kg dry soil.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw