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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 1994-07-28 to 1994-08-11
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Principles of method if other than guideline:
Toxizitaetstest fuer Regenwuermer nach 88/302 EWG
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): WITAMOL 218; Tri-n-C8,C10-alkyltrimellitates
- Physical state: clear liquid
- Analytical purity: 97,5% Trimellitates by volume
- Lot/batch No.: 930914
- Expiration date of the lot/batch: 1994-10-14
- Stability under test conditions: > 1 year

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:
- Method of mixing into soil: The test substance was mixed with silica sand and afterwards added to soil substrate in a plastic bag. Water was added to a theoretical water content of approx. 40% and the substrate was thoroughly mixed

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH&Co. Produktions- und Vertrieb KG, Kapellenstraße 25, Wiesbaden, Germany
- Age at test initiation: at least 2 months
- Weight at test initiation: 301 - 599 mg (mean weight control group: 410 mg; mean weight test substance group: 392 mg)

ACCLIMATION
- Acclimation period: not mentioned

Study design

Study type:
laboratory study
Substrate type:
other: mixture of peat and horse dung (1:1)
Limit test:
yes
Total exposure duration:
14 d
Post exposure observation period:
no

Test conditions

Test temperature:
20 ± 2 °C
pH:
5.6 - 5.7 at the beginning of the test
Moisture:
40% relative to weight of dry substrate
Details on test conditions:
TEST SYSTEM
- Test container (material, size): Makrolon cages with perforated plexiglass covers, size not mentioned
- Amount of soil or substrate: not mentioned
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: not applicable

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): mixture of peat and horse dung (1:1)
- Organic carbon (%): not mentioned
- Maximum water holding capacity (in % dry weigth): approx. 40 %
- CEC: not mentioned
- Pretreatment of soil: not mentioned
- Storage (condition, duration): fresh prepared
- Stability and homogeneity of test material in the medium: not mentioned

OTHER TEST CONDITIONS
- Photoperiod: 24 h
- Light intensity: approx. 400-600 lux

VEHICLE CONTROL PERFORMED: not applicable

TEST CONCENTRATIONS
- Spacing factor for test concentrations: not applicable, limit test
- Range finding study: not performed
Nominal and measured concentrations:
nominal concentration: 1000 mg/kg dry substrate
Reference substance (positive control):
yes
Remarks:
chloracetamide, historical data

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
LC10
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: none
- Total mass of adults at beginning of test: total weight in control 16.4 g; total weight in test substance: 15.7 g
Results with reference substance (positive control):
RESULTS: TEST WITH REFERENCE SUBSTANCE (historical data, carried out from 1994-03-03 to 1994-03-17)
- Concentrations: 0, 10, 30 and 100 mg 2-chloroacetamide/kg dry soil
- Results (mortality):
0 mg/kg: 1/20 (5%) after 7 and 14 days, respectively;
10 mg/kg: 0/20 (0%) after 7 and 14 days, respectively;
30 mg/kg: 18/20 (90%) after 7 and 14 days, respectively;
100 mg/kg: 17/20 (85%) after 7 and 14 days, respectively.

Reported statistics and error estimates:
As no mortality was recorded with the test substance, no statistics were performed.

Any other information on results incl. tables

RESULTS: EXPOSED

- Nominal concentrations: 1000 mg/kg dry soil

- Effect data (Mortality): 0/40 (0%) after 7 days; 0/40 (0%) after 14 days

RESULTS: CONTROL

- Effect data (Mortality): 0/40 (0%) after 7 and 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Not toxic under test conditions at a limit concentration of 1000 mg/kg.
Executive summary:

The acute toxicity of 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters to the earthworm eisenia foetida was determined during a 14 day exposure period according to EEC Methods for Determination of Ecotoxicity, Directive 88/302/EEC. For the limit test with a concentration of 1000 mg/kg dry soil, the test substance was mixed with silica sand and afterwards added to soil substrate. After 7 and 14 days the effects of the test substance on the earthworms' survival were examined.

No mortality occurred at the tested concentration after 7 and 14 days. The 14 day LC50 of WITAMOL 218 to Eisenia foetida was observed to be > 1000 mg/kg dry soil.