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Short-term toxicity to fish

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Description of key information

In all three available studies the test substance was not toxic under test conditions in the range of water solubility. 

Key value for chemical safety assessment

Additional information

Three studies were conducted with the test substances with different fish species.

In the key study the acute toxicity of 1,2,4 -Benzenetricarboxylic acid, mixed decyl and octyl triesters to Zebra fish (Brachydanio rerio) was determined in a 96 -hour static test according to the Commision Directive 92/96/EEC, Annec Part C.1, dated December 29, 1992 and the OECD Guideline for Testing of Chemicals No. 203, adopted July 17, 1992. The test was performed in compliance with Good Laboratory Practice Regulations. Due to the low water solubility limit of the test substance a filtrate of a 1 g/L in tap water (stirring for 18 hours) was tested. Analytical verification was done by means of DOC determination. The measured values of the test solutions after 24 hours deviated more than 20% from the values of the freshly prepared solutions. Therefore the geometric mean (3.0 mg/L) of the these solutions were used for evaluation. Additionally a control was tested in parallel. No lethality of Brachydanio rerio was observed at the tested concentration after 96 hours exposure.

In a second study the LC50 of 1,2,4 -Benzenetricarboxylic acid, mixed decyl and octyl triesters was greater than 1 mg/L, tested with Salmo gaidneri.

In a supporting study the acute toxicity of the test item to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LL50 value of greater than 100 mg/L loading rate WAF. The No Observed Effect Loading rate was 100 mg/L loading rate WAF.

Therefore the test substance was not toxic under test conditions in the range of water solubility.