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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
25. Oct 1956 - 07. Nov 1956
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1961
Report Date:
1961

Materials and methods

Principles of method if other than guideline:
BASF-Test:
50 µL of the test substance were applied to the conjunctival sac of one eye in 2 animals. The animals were observed after 10 min, 1 and 3h on the day of treatment and up to 14 days afterwards. Findings were recorded every 2-3 days. The eyes were not washed out after 24 hours as specified in OECD Guideline 405. The report describes findings after 1 and 24 hours and at the end of the observation period.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.57 kg (mean)

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: The adjacent eye served as saline control.
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 1 drop
Duration of treatment / exposure:
14 days
Observation period (in vivo):
14 days
Number of animals or in vitro replicates:
2

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
other: conjunctiva findings
Time point:
other: after 10 minutes up to 6 days
Reversibility:
not reversible
Remarks:
in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration
Remarks on result:
other: brown-reddish discoloration of the conjunctiva with bloody secretion
Irritation parameter:
other: chemosis
Time point:
other: after 10 minutes up to 6 days
Reversibility:
not reversible
Remarks:
in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration
Remarks on result:
other: slight chemosis
Irritation parameter:
other: corneal findings
Time point:
other: after 10 minutes up to 6 days
Reversibility:
not reversible
Remarks:
in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration
Remarks on result:
other: Opacity
Irritation parameter:
other: Eye lense
Time point:
other: after 10 minutes up to 6 days
Reversibility:
not reversible
Remarks:
in fact, at the end of the observation period evaluation of the eyes was not possible because of suppuration
Remarks on result:
other: welling up of the eye lenses

Any other information on results incl. tables

The application of the test substance caused severe, irreversible corrosion. After 10 min up to 6 days brown-reddish discoloration of conjunctiva, bloody secretion, opacity and slight chemosis was noted. At the end of the observation period evaluation of the eyes due to suppuration not possible. Welling up of eye lenses.

Applicant's summary and conclusion