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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Description of key information

The substance is acutely harmful to aquatic algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
34 mg/L
EC10 or NOEC for freshwater algae:
26 mg/L

Additional information

 The effects of 3 -aminopropyldimethylamine (DMAPA, CAS 109-55-7) on freshwater algae have been assessed using data from a growth inhibition test with DMAPA according to DIN 38412-9 (BASF AG, 1987) and read-across data from three tests with the structurally similar substance 3 -aminopropyldiethylamine (DEAPA, CAS 104-78-9).


The determination of the growth inhibition of the fresh algae Pseudokirchneriella subcapitata exposed to DEAPA for a duration of 72 h was performed following the method OECD 201 and according to GLP (Arkema, 2000). Algae were exposed to the following nominal concentrations for 72 h: 0, 4.77, 7.63, 12.21, 19.53, 31.25 and 50 mg/L. The final concentrations of DEAPA were maintained within the designated limit of 80% of the initial concentrations in non-inoculated flasks. Therefore, the effect concentrations have been calculated using the nominal concentrations. The validity criteria of the study were fulfilled. The ErC50 (72 h) was determined to be 34 and the ErC10 (72 h) was 26 mg/L. These data were selected as key values for the assessment.


In a supporting read-across study, acute toxicity of DEAPA to Desmodesmus subspicatus was investigated according to German Industrial Standard DIN 38 412-9 (BASF AG, 1990). The ErC50 (72 h) was determined to be between 100 and 150 mg/L.

The concentrations were not analytically verified but DEAPA is expected to be stable in water as shown in the key study. Moreover, DEAPA is miscible in water, its vapor pressure is low and it is not subject to hydrolysis.

Another test with DEAPA was conducted according to German Industrial Standard DIN 38 412-9 (BASF AG, 1990). The stock solution of DEAPA was neutralised to pH 7. In this study, the ErC10 (72 h) and ErC50 (72 h) were determined to be 3450 mg/L and 7120 mg/L, respectively. These results indicate that the toxicity of DEAPA is at least partly related to the pH


The available results of the study conducted with the target substance DMAPA (CAS 109-55-7) itself fit in with the read-across data of the studies with the structurally analogous substance DEAPA (CAS 104-78-9). The 72-h static test with Scenedesmus subspicatus was performed according to the German Industrial Standard Test Guideline DIN 38412 -9 (BASF AG, 1987). The results were recalculated using ToxRatPro v2.09 (2009). The concentrations were not analytically verified but DMAPA is also expected to be stable in water due to the high water solubility (1E+06 mg/L; HSDB, 1993), the moderate vapour pressure (5.9 hPa; BASF AG, 1974) and a low Henry's law constant (6.71E-04 Pa*m^3/mol; BASF SE, 2016). The re-evaluation showed that the validity criteria of the recent guideline OECD 201 were not fully met, but are in agreement with the above mentioned results of the structurally similar substance DEAPA. After 72 hours an EbC50 of 53.5 mg/L and an EbC10 of 43.0 mg/L were estimated for the biomass, related to the nominal concentration. The effect values estimated for the growth rate were ErC50 (72 h) = 64.3 mg/L and ErC10 (72 h) = 48.4 mg/L, related to the nominal concentration.


In an additional marine study performed according to ISO 10253 using Skeletonema costatum as test species a 72-h ErC50 of 55 mg/L was determined, based on nominal test concentrations.


Based on the available results, it can be concluded that DMAPA is acutely harmful to aquatic algae.