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Toxicity to Soil Macroorganisms


PR002


Under the conditions of this study, the test item did not induce mortality, pathological symptoms or changes in the behaviour of adult earthworms after 28 days of exposure at the nominal limit test item concentration of 1000 mg/kg soil dw. The adult earthworm biomass increased in the control and at the limit test item concentration without any statistically significant differences. After eight weeks of exposure the earthworm reproduction at the limit test item concentration did not statistically significantly differ from the pooled control. As there had been no statistically significant differences determined between the control and the solvent control for the body weight and the reproduction rate, the controls were pooled.


Thus, the No Observed Effect Concentration (NOEC) for the test item summarizing the endpoints mortality, body weight and reproduction of Eisenia fetida fetida after 8 weeks of exposure in artificial soil was determined to be 1000 mg /kg soil dry weight.


 


Toxicity to Terrestrial Plants


PR112


The phytotoxicity of the test item Pigment Red 112 to three terrestrial plant species was determined in a seedling emergence test over a period of 21 days according to OECD 208. The test was conducted with the nominal test item concentration 1000 mg/kg soil dry weight (DW). Test systems were one monocotyledon, oats (Gramineae), and two dicotyledons, rape (Brassicaceae) and soybean (Leguminosae). The toxic effects of the test item were determined on day 7, 14 and 21 by visual observations (number of emerged seedlings and phytotoxic effects) and on day 21 by shoot height and shoot fresh weight determination. The inhibition of shoot height, shoot fresh weight and rate of emergence was calculated in relation to the untreated control. The test item did not cause any effects > 25 % on the shoot height, the shoot fresh weight and the rate of emergence of the tested plant species at the tested concentration. After 21 days no test item related phytotoxic effects were observed for the tested plant species.


 


 

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