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Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Study performed in accordance with the relevant guidelines but not under the conditions of GLP. Substance is provided as supporting data only as substance is not considered to be toxic to terrestrial organisms and as such an investigation of earthworm toxicity is not required.

Data source

Reference
Reference Type:
publication
Title:
A comparison of the relative toxicity of bone meal and other P sources used as remedial treatments to the earthworm
Author:
Atuah L & Hodson ME
Year:
2011
Bibliographic source:
Pedobiologica - International Journal of Soil Biology

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium dihydrogenorthophosphate
EC Number:
231-913-4
EC Name:
Potassium dihydrogenorthophosphate
Cas Number:
7778-77-0
Molecular formula:
H3O4P.2K
IUPAC Name:
potassium dihydrogen phosphate
Details on test material:
- Name of test material (as cited in study report): potassium hydrogen phosphate (KH2PO4)

Test substrate

Vehicle:
no
Details on preparation and application of test substrate:

- Controls: unamended soil

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: Blades biological Ltd, Cowden, Edenbridge, Kent, UK
- Age at test initiation (mean and range, SD): no data
- Weight at test initiation (mean and range, SD): 340 +/- 3.7 mg

ACCLIMATION
- Acclimation period: no data

Study design

Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
28 d

Test conditions

Test temperature:
20°C
pH:
7-7.2
Moisture:
60% water holding capacity
Details on test conditions:
TEST SYSTEM
- Test container (material, size): no data
- Amount of soil or substrate: 500 g
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Jetting Loam - bought
- Soil texture (if natural soil): sieved to less than 2mm

Properties are similar to the OECD standard soil.

OTHER TEST CONDITIONS
- Photoperiod: performed in dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
Nominal and measured concentrations:
15,000 mg P/kg with concentrations decreasing by a factor of 1.8 downwards to 441 mg P/kg
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 3 500 other: mg P/ kg
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Duration:
28 d
Dose descriptor:
LC50
Effect conc.:
> 3 500 other: mg P / kg
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
LC50 (14 day and 28 day values) of potassium dihydrogen phosphate was found to be > 3500 mg P / kg.