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Based on a combination of test data tripropylene glycol presents a low hazard concern for the aquatic environment.

One non-GLP compliant guideline study (OECD 203) is available for tripropylene glycol. The 96-h LC50 value is >1,000 mg/l (nominal concentration) in Oryzias latipes in a semi-static test. One non-GLP compliant guideline study (OECD 202) is available for tripropylene glycol. The 24-h EC50 value is > 1,000 mg/l (nominal concentration) in Daphnia magna in a static test. One non-GLP compliant guideline study (OECD 202) is available for tripropylene glycol. The 21-d NOEC value is >1,000 mg/l (nominal concentration) in Daphnia magna in a semi-static test. One non-GLP compliant guideline study (OECD 201) is available for tripropylene glycol. The 72-h EbC50 value and 72-h NOEbC value is >1,000 mg/l (nominal concentration) in Pseudokirchnerella subcapitata (reported as Selenastrum capricornutum) (OECD, 1994).

In an OECD209 test, the 3h-EC50 was > 1000 mg/L (nominal) and the 3h-NOEC was 1000 mg/L (Harlan Laboratoried, Ltd., 2010).

Long-term exposure of fish is not considered relevant as the substance is readily biodegradable. Based on the fact that the short-term toxicity test shows very low toxicity to fish, the experimental 21d-NOEC to Daphnia magna is >1000 mg/l (OECD, 1994) and the QSAR for long-term toxicity to fish is high (30-d ChV is 1631 mg/l), chronic toxicity to fish is not expected. Therefore, the long-term toxicity test with fish is not considered necessary.