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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The test was carried out according to the appropriate OECD test guideline and in compliance with GLP. However, no chemical analysis was performed.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
EC Number:
222-048-3
EC Name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
Cas Number:
3327-22-8
Molecular formula:
C6H15ClNO.Cl
IUPAC Name:
3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium chloride
Constituent 2
Reference substance name:
(3-chloro-2-hydroxypropyl)trimethylammoniumchloride
IUPAC Name:
(3-chloro-2-hydroxypropyl)trimethylammoniumchloride
Details on test material:
65.44 % 3-chloro-2-hydroxypropyltrimethylammoniumchloride was used. The purity of the QUAB 188 was 96.64 %.

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Nominal and measured concentrations:
Nominal concentrations 1.000, 1.800, 3.200, 5.600 and 10.000 of QUAB 188 mg/L
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
2 094 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4 128 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
> 6 544 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality (fish)
Reported statistics and error estimates:
The LC50 values and their confidence interval were calculated by means of a parametric model developed by Kooijman (1981).

Any other information on results incl. tables

Sublethal observations / clinical signs:

NOEC 3200 mg (2094 mg/l), but in concentration 1800 mg/l (1178 mg/l) one fish died (the death is assumed to be accidental).

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
A 96 h EC50 value of 4128 mg a.i./L has been determined for the effects of the test substance on mortality of B. rerio.