Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 207-938-1 | CAS number: 502-44-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 72 hours, 4 to 7 March 2003
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline and GLP-compliant study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II) (January 2012)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: Caprolactone Consortium (BASF, Daizel, Dow and Solvay)/ Batch number: U 05175
- Purity test date: 99.95 %
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: at room temperature
- Stability: unlimited. Stability of the test substance under storage conditions over exposure period was guaranteed by the sponsor and the sponsor holds this responsability
- Aggregate state/appearance: liquid /clear, colourless
- Water-solubility: soluble
- Homogeneity: homogeneous (sponsor data)
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken at 0 hours (fresh media) and 72 hours (expired media).
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was stirred in deionised water for about 10 minutes at approximately 20 ± 2 °C.
- The nominal concentration of the stock solution was 5000.2 mg/I . By diluting this stock solution the following nominal concentrations (mg/I) were prepared: control, 4000, 1600, 640, 256 and 102 mg/l
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): The stock solution appears clear and colourless - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name:
- Strain: CHODAT SAG 86.81
- Source (laboratory, culture collection): SAG (Collection of algal cultures in Göttingen)
Pre-culture techniques
- Seed-culture: a seed culture was incubated for 4 days at 23 °C ± 2 °C. Final cell density: 89 x 10-4 cells/ml
- Pre-culture: seed-culture was taken to inoculate pre-culture (initial cell density: 1 x 10-4 cells/ml}. The pre-culture was incubated for 3 days at 23 °C ± 2 °C. Final cell density: 126 x 104 cells/ml. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- none
- Post exposure observation period:
- not applicable
- Hardness:
- Not applicable
- Test temperature:
- 23 ± 2 °C
- pH:
- 6.9 - 8.2
- Dissolved oxygen:
- Not applicable
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal: 102, 256, 640, 1600, 4000 mg/L
Measured (mean of 2): 93, 260, 664, 1693, 4204 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flasks (nominal volume 250 ml) plugged with gas permeable siliconsponge caps
- Test volume: 100 ml
- Initial cells density: 1 x 104
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Intervals of water quality measurement:
- Measurement of temperature: continuously during the whole test period in the climate chamber
- Measurement of pH-values: after 0 h and 72 h in an additional uninoculated replicate and after 72h in the inoculated replicate
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Light intensity and quality: about 60 - 120 uE/(m2s) at a wave length of 400 - 700 nm. Artificial light, type universal white (OSRAM L 25), permanent illumination.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: after 72 h in a counting chamber (Neubauer improved) from a control vessel
- Chlorophyll measurement: in vivo chlorophyll-a-fluorescence (pulsed excitation with light flashes having a wavelength of 435 nm) - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1 217 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2 616 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 256 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: biomass and growth rate
- Details on results:
- none
- Reported statistics and error estimates:
- Calculation of the integral of biomass growth over the total duration of the test for each concentration level and comparison of values of treated samples in relation to untreated samples.
Calculation of the growth rate over the total duration of the study for each concentration level and comparison of values of treated samples in relation to untreated samples.
The EC values were calculated (linear regression analysis) from the concentration-response relationship.
The LOEC was determined by comparing the means of the calculated biomass or growth rate of the various concentration levels with the control. The Dunnett's One-tailed T test was carried out at a 95% significance level.
The NOEC is the tested concentration immediately below the LOEC. - Validity criteria fulfilled:
- yes
- Conclusions:
- The 72-hour ErC50 (growth rate), EbC50 (integrated biomass) for D. subspicatus exposed to the test material under static conditions were 2616 and 1217 mg/L, respectively. The NOEC for biomass and growth rate was 256 mg/l.
- Executive summary:
Desmodesmus subspicatus was exposed to test material at nominal concentrations of 102, 256, 640, 1600 and 4000 mg/L in a static 72 -hour toxicity test carried out according to OECD 201 guidelines. The study was reliable (Klimish 1) and conducted by GLP standards. The test design included an untreated control comprising untreated algal growth medium only. Analytical concentrations were verified at test initiation and termination (72h) and measured concentrations were 91 - 106% of nominal. The 72 -hour ErC50 and EbC50 endpoints determined were 2616 and 1217 mg/L, respectively. The corresponding 72hr NOEC, based on inhibition of specific growth rate or inhibition of biomass was 256 mg/L.
Reference
Results of the definitive test are compiled below:
Measured fluorescence (relative units)
Time (h) |
Flask number |
Concentration (mg/L) |
|||||
0 (control) |
4000 |
1600 |
640 |
256 |
102 |
||
0 |
1 |
30 |
31 |
31 |
32 |
31 |
33 |
2 |
31 |
32 |
34 |
33 |
31 |
35 |
|
3 |
30 |
32 |
32 |
31 |
31 |
34 |
|
no |
0 |
0 |
0 |
0 |
0 |
0 |
|
24 |
1 |
130 |
98 |
130 |
130 |
150 |
130 |
2 |
130 |
95 |
130 |
140 |
140 |
140 |
|
3 |
130 |
95 |
130 |
140 |
150 |
140 |
|
no |
0 |
0 |
0 |
0 |
0 |
0 |
|
48 |
1 |
660 |
180 |
440 |
630 |
730 |
660 |
2 |
700 |
190 |
420 |
670 |
750 |
680 |
|
3 |
680 |
190 |
430 |
660 |
780 |
680 |
|
no |
0 |
0 |
0 |
0 |
0 |
0 |
|
72 |
1 |
3450 |
170 |
930 |
2720 |
3330 |
3380 |
2 |
3500 |
150 |
780 |
2450 |
3800 |
3590 |
|
3 |
3180 |
150 |
730 |
2350 |
3970 |
3630 |
|
no |
0 |
0 |
0 |
0 |
0 |
0 |
no = not inoculated
Cell density after 0 h, 24 h, 4$ h and 72 h
Time (h) |
Flask number |
Concentration (mg/L) |
|||||
0 (control) |
4000 |
1600 |
640 |
256 |
102 |
||
0 |
1 |
1.5 |
1.6 |
1.6 |
1.6 |
1.6 |
1.7 |
2 |
1.6 |
1.6 |
1.7 |
1.7 |
1.6 |
1.8 |
|
3 |
1.5 |
1.6 |
1.6 |
1.6 |
1.6 |
1.7 |
|
mean |
1.5 |
1.6 |
1.6 |
1.6 |
1.6 |
1.7 |
|
24 |
1 |
6.6 |
5.0 |
6.6 |
6.6 |
7.6 |
6.6 |
2 |
6.6 |
4.7 |
6.6 |
7.1 |
7.1 |
7.1 |
|
3 |
6.6 |
4.8 |
6.6 |
7.1 |
7.6 |
7.1 |
|
mean |
6.6 |
4.9 |
6.6 |
7.0 |
7.5 |
7.0 |
|
48 |
1 |
33.6 |
6.2 |
22.4 |
32.1 |
37.2 |
33.6 |
2 |
35.7 |
9.7 |
21.4 |
34.2 |
38.2 |
34.7 |
|
3 |
34.7 |
9.7 |
21.9 |
33.6 |
39.8 |
34.7 |
|
mean |
34.7 |
9.5 |
21.9 |
33.3 |
38.4 |
34.3 |
|
72 |
1 |
175.9 |
8.7 |
47.4 |
138.7 |
169.8 |
172.3 |
2 |
178.4 |
7.6 |
39.8 |
124.9 |
193.7 |
183.0 |
|
3 |
162.1 |
7.6 |
38.7 |
119.8 |
202.4 |
185.1 |
|
mean |
172.1 |
8.0 |
42.0 |
127.8 |
118.6 |
180.1 |
Calculation of biomass and inhibition of biomass after 72h
Parameter |
Flask number |
Concentration (mg/L) |
|||||
0 (control) |
4000 |
1600 |
640 |
256 |
102 |
||
Biomass (rel) |
1 |
124 |
15 |
49 |
104 |
126 |
122 |
2 |
128 |
14 |
44 |
100 |
138 |
129 |
|
3 |
119 |
14 |
44 |
97 |
145 |
130 |
|
mean |
120 |
14 |
45 |
100 |
136 |
127 |
|
Inhibition (%) |
|
0.0 |
88.4 |
63.2 |
19.0 |
-10.3 |
-2.9 |
Calculation of growth rate and inhibition of growth rate after 72h
Parameter |
Flask number |
Concentration (mg/L) |
|||||
0 (control) |
4000 |
1600 |
640 |
256 |
102 |
||
Growth rate (rel) |
1 |
0.066 |
0.024 |
0.047 |
0..062 |
0.065 |
0.064 |
2 |
0.066 |
0.021 |
0.044 |
0.060 |
0.067 |
0.064 |
|
3 |
0.065 |
0.021 |
0.044 |
0.060 |
0.067 |
0.065 |
|
mean |
0.065 |
0.022 |
0.045 |
0.061 |
0.066 |
0.064 |
|
Inhibition (%) |
|
0.0 |
66.1 |
31.4 |
7.5 |
-1.4 |
1.4 |
Description of key information
The 72-hour ErC50 (growth rate), EbC50 (integrated biomass) for D. subspicatus exposed to the test material under static conditions were 2616 and 1217 mg/L, respectively. NOEC for biomass and growth rate was 256 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 1 217 mg/L
- EC10 or NOEC for freshwater algae:
- 256 mg/L
Additional information
Desmodesmus subspicatuswas exposed to test material at nominal concentrations of 102, 256, 640, 1600 and 4000 mg/L in a static 72 -hour toxicity test was carried out according to OECD 201 guidelines. The study was reliable (Klimish 1) and conducted by GLP standards. The test design included an untreated control comprising untreated algal growth medium only. Analytical concentrations were verified at test initiation and termination (72h) and measured concentrations were 91 - 106% of nominal. The 72 -hour ErC50 and EbC50 endpoints determined were 2616 and 1217 mg/L, respectively. The corresponding 72hr NOEC, based on inhibition of specific growth rate or inhibition of biomass, was 256 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.