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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Not reported
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Guideline study with acceptable restrictions. No GLP or analysis of exposure media and no description of the test substance.
Principles of method if other than guideline:
Not described
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
no data
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:

TEST ORGANISM

- Common name: Rainbow trout

- Source: Fender's Fish Hatchery

- Feeding during test: no data

ACCLIMATION

- Acclimation period: A minimum of two weeks

- Acclimation conditions: same as test

- Type and amount of food: not reported
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
Soft water
Test temperature:
12ºC
pH:
no data
Dissolved oxygen:
no data
Salinity:
Not applicable
Nominal and measured concentrations:
Nominal concentrations: 0(Control), 100, 320 and 1000 mg/l
Details on test conditions:
TEST SYSTEM

- Test vessel:

- Type: open

- Material: Polyethylene lined

- Aeration: no data

- Renewal rate of test solution (frequency/flow rate): no data

- No. of organisms per vessel: 10

- No. of vessels per concentration (replicates): 1

- No. of vessels per control (replicates): 1


TEST MEDIUM / WATER PARAMETERS

- Source/preparation of dilution water: Reconstituted soft water (EPA 660/3-75-009)

- Culture medium different from test medium: no

- Intervals of water quality measurement: no data


OTHER TEST CONDITIONS

- Adjustment of pH: no data

- Photoperiod: no data

- Light intensity: no data


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): daily mortality


TEST CONCENTRATIONS

- Spacing factor for test concentrations: 3.2

- Range finding study: no data
Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
191 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 95% confidence interval 125-281 ppm
Details on results:
- Mortality of control: 10% (1 fish)

- Other adverse effects control: none
Reported statistics and error estimates:
The LC50 value was calculated by probit analysis based on Finney's Method (Statistical Methods in Biological Assay, 1952).
Sublethal observations / clinical signs:

Table 1. Test results

 Nominal test concentration (mg/l)  Percentage mortality after 24 hours   Percentage mortality after 48 hours   Percentage mortality after 72 hours  Percentage mortality after 96 hours 
 0 (Control)  0  0  10  10
 100  0  0  0  0
 320  30  100  100  100
 1000  90  100  100  100
Validity criteria fulfilled:
yes
Conclusions:
A 96-hour L50 value of 191 mg/l and NOEC of 100 mg/l have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss. It is likely that the test organism were primarily exposed to the hydrolysis product of the test substance.

Description of key information

96 hour LC50: 191 mg/L, for effects of the test substance on mortality of Oncorhynchus mykiss. It is likely that the test organism were primarily exposed to the hydrolysis product of the test substance. The LC50 is equivalent to 137 mg/L when expressed in terms of the silanol hydrolysis product vinylsilanetriol.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
137 mg/L

Additional information

A 96 hour LC50 value of 191 mg/L (nominal concentration) has been determined for the effects of the test substance on mortality of Oncorhynchus mykiss (Dow Corning Corporation 1979). It is likely that the test organisms were primarily exposed to the hydrolysis product of the test substance. The results may be expressed in terms of the silanol hydrolysis product, by adjusting for the respective molecular weights: MW hydrolysis product/MW parent * E(L)C50 or NOEC value, thus 106.15/148.24 * LC50 of 191 mg/L = 137 mg/L as vinylsilanetriol.

The results of this test were selected in preference to those of two available studies with higher reliability scores, because a definitive (although comparable) LC50 value was determined. Other available test results were less reliable or gave greater-than limit results. The following results are from studies included as supporting data:

96-hour LC50 >1000 mg/L for effects of the test substance on mortality of Lepomis macrochirus (Dow Corning Corporation, 1979).

96-hour LC50 >100 mg/L for effects of the test substance on mortality of Danio rerio (Scholz, 1995).

96-hour LC50 >100 mg/L for effects of the test substance on mortality of Oncorhynchus mykiss (Bement, 2001).

96-hour LC50 >92.2 mg/L for effects of the test substance on mortality of Oryzias latipes (MOE, 2006).

A further study, for which the reliability could not be ascertained, is also included in the dataset: LC50 >2000 mg/L for effects of the test substance on mortality of Pimephales promelas (Chandra, 1997).