[2-(acryloyloxy)ethyl]trimethylammonium chloride

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

18/07/1991-20/07/1991

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented report of a guideline study conducted to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

The dissolved oxyegen concentration was 4% below the required minimum of 60% of the air saturation value at the beginning of the test and 8% after 24 hours and was not measured at 48 hours.

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

EC Number:

256-176-6

EC Name:

[2-(acryloyloxy)ethyl]trimethylammonium chloride

Cas Number:

44992-01-0

Molecular formula:

C8H16NO2.Cl

IUPAC Name:

Trimethyl[2-(prop-2-enoyloxy)ethyl]azanium chloride

Details on test material:

- Name of test material: Adame-Quat (2-(acryloyloxy)-N,N,N-trimethylethanaminium chloride)
- CAS number: 44992-01-0
- Manufacturing code: B4846
- Physical state: liquid
- Colour: clear liquid
- Analytical purity: 80 % aqueous solution
- Lot/batch No.: 40034237
- Storage condition of test material: at 4°C in the dark
- pH-value: 6.56

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

The resulting dilution water had total hardness of 2.619 mmol/l calcium and magnesium ions. Carbonate content was 0.760 mmol/L. Before use the dilution water was filtered through an active carbon tilter in order to eliminate chlorinated hydrocarbons. The pH-value was 7.94. All these parameters were within the pre-set ranges.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: no data
- Source: Stockhausen laboratory
- Age at study initiation: not older than 25 hours
- Method of breeding: Beakers with a nominal capacity of 2 litres, filled with 1400 ml dilution water. Between 15 and 20 adults held in each.
- Feeding during test: during holding
- Food type: Algae: Scenedesmus subspicatus
- Amount and Frequency: daily with an appropriate amount of algae which could be roughly consumed per day.
ACCLIMATION
- Acclimation period: not required
- Acclimation conditions: same as test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

No

Test conditions

Hardness:

2.619 mmol/l calcium and magnesium ions
0.760 mmol/l carbonate

Test temperature:

Day 0: 20.2°C
Day 1: 20.4°C

pH:

day 0: 7.53
day 1: 7.62

Dissolved oxygen:

Day 0: 5.1 mg/L and 56 %
Day 1: 4.7 mg/L and 52 %

Nominal and measured concentrations:

Nominal concentrations: 5; 10; 20; 40; 80; 160; 320 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass beakers (with approximately 30 ml test solution)
- Type: open
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration: 5
- No. of vessels per vehicle control (replicates): 5
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: the dilution water was prepared separately in a silicon sealed glass tank by mixing de-chlorinated tap water from a municipal source with demineralised water a certain amount of sulphuric acid. The resulting dilution water had total hardness of 2.619 mmol/l calcium and magnesium ions. The carbonate content was 0.760 mmol/L.
Before usage the dilution water was filtered through an active carbon filter in order to eliminate chlorinated hydrocarbons.
OTHER TEST CONDITIONS
- Photoperiod: natural light-dark cycle and additional artificial light-dark cycle of 16 to 8 hours
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobilization at 24 hours and 48 hours post exposure

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

40 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

320 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mobility

Results with reference substance (positive control):

- Results with reference substance valid? Yes (potassium dichromate)
- Toxicity range required : EC50 = 0.9 - 1.9 mg/L
- Measured toxicity: 1.45 mg/L

Any other information on results incl. tables

Nominal Concentration (mg/L)	Number of Daphnia	Immobilized Daphnia
		After 24 hours		After 48 hours
		Number	Cumulative Frequency (%)	Number	Cumulative frequency (%)
0	25	0	0	1	4
5	25	0	0	1	4
10	25	0	0	1	4
20	25	0	0	2	8
40	25	0	0	1	4
80	25	0	0	9	36
160	25	4	16	11	60
320	25	25	100	-	100

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The test substance, Adam-Quat MC, was considered to be only slightly toxic to Dapnia magna, with regard to the swimming ability. Nevertheless ADAMQUAT MC is not classified harmful to aquatic invertebrates. The lowest concentration which did not cause swimming inhibition was 40 mg Adam-Quat MC per litre. Therefore the EC0 is 40 mg/l. The EC 50 was calculated to be 183 mg/L after 24 hours and 116 mg/l after 48 hours according to the EPA computer programme.