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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
18/08/1994-16/09/1994
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented report of a guideline study conducted to GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report date:
1994

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
System was purged of CO2 after addition of the test substance and before addition of the inoculum in order to lower the endogenous respiration. Inorganic carbon (IC) was estimated by subtracting total organic carbon (TOC) from total carbon (TC).
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
[2-(methacryloyloxy)ethyl]trimethylammonium chloride
EC Number:
225-733-5
EC Name:
[2-(methacryloyloxy)ethyl]trimethylammonium chloride
Cas Number:
5039-78-1
IUPAC Name:
2-(methacryloyloxy)-N,N,N-trimethylethanaminium chloride
Details on test material:
- Name of test material (as cited in study report): MADQUAT MC 75 (chlorure de methacryloxyethyltrimethylammonium )
- Molecular formula (if other than submission substance): C9H18NO2.Cl
- Molecular weight (if other than submission substance): 207.5
- Smiles notation (if other than submission substance): O=C(OCCN(Cl)(C)(C)C)C(=C)C
- InChl (if other than submission substance): InChI=1S/C9H18NO2.ClH/c1-8(2)9(11)12-7-6-10(3,4)5;/h1,6-7H2,2-5H3;1H/q+1;/p-1
- Physical state: clear yellowish liquid
- Analytical purity: 75 %
- Purity test date: 1994/07/25
- Lot/batch No.: M 67 Bis

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Domestic sewage treatment plant, Versailles, France
- Storage length: 24 h
- Preparation of inoculum for exposure: 4000g centrifugation for 20 mn at 20°C . Then adjustment to a concentration factor of 40.
- Pretreatment: aeration
- Concentration of sludge: 1%
- Initial cell/biomass concentration: 1,25 x 10 3 bact/ml,
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
ca. 48.7 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: 1% = 1.25 x 10 3 bact/ml
- Test temperature: 22 +/- 2°C
- pH: 7.4
- pH adjusted: no
- CEC (meq/100 g): 41
- Aeration of dilution water: air flow
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 3000 ml glass flasks containing 1500 ml test medium
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: air flow
- Measuring equipment: Oxymetre, conductimetre, pH-rnetre : multifonctions apparatus Jenway 3410 , Carlo Erba automatic CO2 analyser, Dissoved CO2 analyser MAIHAK TOCOR 100
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: Soude 5 M, hydroxyde de baryum 0,0125 M, acide chlorhydrique 0,05 M
SAMPLING
- Sampling frequency: on days 1, 4, 5, 8, 11, 15, 18, 22, 25, 28, 29
- Sampling method: CO2
STATISTICAL METHODS: none
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
Difference between the two replicates was less 20% at the 10 day window.
The biodegradation of the reference substance was greater than 70% in 14 days.
% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
46
Sampling time:
4 d
Parameter:
% degradation (CO2 evolution)
Value:
61
Sampling time:
8 d
Parameter:
% degradation (CO2 evolution)
Value:
70
Sampling time:
15 d
Parameter:
% degradation (CO2 evolution)
Value:
71
Sampling time:
21 d
Parameter:
% degradation (CO2 evolution)
Value:
69
Sampling time:
28 d
Details on results:
Degradation peaked at 71% after 22 days. The lag phase (time to attain 10% degradation) was less than 5 days and degradation attained 70% 10 days later. The abiotic sterile control demonstrated that 33% abiotic degradation (physical or chemical) took place.

BOD5 / COD results

Results with reference substance:
Biodegradation was greater than 60% in 14 days.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
MADAMQUAT MC is considered to be readily biodegradable (69% in 28 days).