Amines, C16-18 and C18-unsatd. alkyl

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

yes

Remarks:

no detailed documentation

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Amines, coco alkyl

EC Number:

262-977-1

EC Name:

Amines, coco alkyl

Cas Number:

61788-46-3

IUPAC Name:

61788-46-3

Details on test material:

- Name of test material (as cited in study report): Genamin CC 100 D ("Genamin CC 100 D-Umarbeitung")

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

activated sludge of a predominantly domestic sewage

Details on inoculum:

- Source of inoculum: Activated sludge from domestic STP Hildesheim, Germany
- Preparation of inoculum for exposure: After settling the sludge was washed twice with dechlorinated (autoclaved) tap water
- Initial biomass concentration: activated sludge dry weight: 2.3 g/L

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

3 h

Test conditions

Test temperature:

21.9°C

Nominal and measured concentrations:

Nominal concentrations: 5, 10, 20, 40, 80 mg/L

Details on test conditions:

TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
-Culture medium: synthetic sewage feed as described in OECD 209
- Dilution water: Dechlorinated tap water
- Intervals of water quality measurement: continuous measurement of oxygen content using a oxygen electrode


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : inhibition of respiration rate

Reference substance (positive control):

yes

Remarks:

potassium dichromate (60 mg/L)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Remarks on result:

other: 95% CL: 5.7-34.6 mg/L

Effect concentrations 2

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

5.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

7.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Effect concentrations 4

Duration:

3 h

Dose descriptor:

other: EC80

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The controls had a deviation of 10%, thus the test is valid.

Results with reference substance (positive control):

- Results with reference substance valid? yes, 57.9% inhibition of respiration rate

Any other information on results incl. tables

Table 1: Respiration inhibition of Genamin CC 100 D („Umarbeitung“)

	Test concentrations (mg/L)	Respiration rate R (mgO2/L*h)	Inhibition (%)
Main test	5 10 20 40 80	27.6 17.4 4.5 1.6 1.2	3.2 38.9 84.2 94.4 95.8
Reference	60	12.0	57.9
Control	K1 K2	27.0 30.0