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Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report date:
1989

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
no detailed documentation
GLP compliance:
no

Test material

Constituent 1
Reference substance name:
Amines, coco alkyl
EC Number:
262-977-1
EC Name:
Amines, coco alkyl
Cas Number:
61788-46-3
IUPAC Name:
61788-46-3
Details on test material:
- Name of test material (as cited in study report): Genamin CC 100 D ("Genamin CC 100 D-Umarbeitung")

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Source of inoculum: Activated sludge from domestic STP Hildesheim, Germany
- Preparation of inoculum for exposure: After settling the sludge was washed twice with dechlorinated (autoclaved) tap water
- Initial biomass concentration: activated sludge dry weight: 2.3 g/L

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h

Test conditions

Test temperature:
21.9°C
Nominal and measured concentrations:
Nominal concentrations: 5, 10, 20, 40, 80 mg/L
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
-Culture medium: synthetic sewage feed as described in OECD 209
- Dilution water: Dechlorinated tap water
- Intervals of water quality measurement: continuous measurement of oxygen content using a oxygen electrode


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : inhibition of respiration rate
Reference substance (positive control):
yes
Remarks:
potassium dichromate (60 mg/L)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CL: 5.7-34.6 mg/L
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
5.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
7.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The controls had a deviation of 10%, thus the test is valid.
Results with reference substance (positive control):
- Results with reference substance valid? yes, 57.9% inhibition of respiration rate

Any other information on results incl. tables

Table 1: Respiration inhibition of Genamin CC 100 D („Umarbeitung“)

 

Test concentrations (mg/L)

Respiration rate

R (mgO2/L*h)

Inhibition (%)

Main test

5

10

20

40

80

27.6

17.4

4.5

1.6

1.2

3.2

38.9

84.2

94.4

95.8

Reference

60

12.0

57.9

Control

K1

K2

27.0

30.0

 

Applicant's summary and conclusion

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