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EC number: 268-219-6 | CAS number: 68037-95-6 This substance is identified by SDA Substance Name: C16-C18 and C18 unsaturated alkyl amine and SDA Reporting Number: 11-029-00.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Amines, coco alkyl
- EC Number:
- 262-977-1
- EC Name:
- Amines, coco alkyl
- Cas Number:
- 61788-46-3
- IUPAC Name:
- 61788-46-3
- Details on test material:
- - Name of test material (as cited in study report): Cocoalkylamine
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution was prepared with natural river water. Dispersion carried out with: 30 min ultrasound at 40 °C
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: Green algae
- Strain: Desmodesmus subspicatus CHODAT SAG 86.81
- Source: The algae culture was optained from the Institut of plant physiology, University of Göttingen, Germany;
- Method of cultivation: Fresh stock solutions were prepared every month on Z-agar. Light intensity amounted 35-70 µE/m²
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 91.3 mg CO3/L
- Test temperature:
- 23 ± 2 °C
- pH:
- 8.08 - 8.15
- Nominal and measured concentrations:
- Nominal concentrations: control, 0.03, 0.06, 0.13, 0.25, 0.5 and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: 250 mL-Erlenmayer-flasks with 100 mL test volume
- Initial cells density: 9000-10000 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: Yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes
- Intervals of water quality measurement: cell density was measured every 24 hours. Temperature was measured permanently and the pH value was determinated at the beginning and the end of the test.
OTHER TEST CONDITIONS
- Photoperiod: Permanent illumination (24h)
- Light intensity and quality: 60-120 µE/m²*s
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: fluorimeter and microscopic evaluation - Reference substance (positive control):
- yes
- Remarks:
- potassium dicromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.06 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
After 72 h algae were transferred from the nominal dosage levels 0.13 -1.0 mg/L to fresh untreated medium and allowed to grow for further 5 -11 days under test conditions. The test item effect was observed to be reversible at these concentration levels (exept one of three replicates of the highest test concentration of 1 mg/L, where no growth could be detected with 11 d of incubation).
Table 1: Concentration of the test item:
Nominal test item conc. [mg/L]
|
Meas. conc. [mg/L]after 0 h |
Meas. conc. [mg/L]after 3 d |
20.00 |
16.1 |
12.9 |
1.00 |
(18.4) |
< detection limit |
Control |
< detection limit |
< detection limit |
Applicant's summary and conclusion
- Conclusions:
- In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.
Inhibition effects were found to be reversible up to 1.0 mg/L (all effect levels are given as nominal concentrations) - Executive summary:
In this study Cocoalkylamine was found to inhibit the growth of the freshwater green alga Desmodesmus subspicatus at concentrations > 0.06 mg/L (biomass growth) and > 0.13 mg/L (growth rate). The EC50 values with 95 % confidence intervals for inhibition of biomass growth (EbC50) and specific grothw rate (ErC50) after 72h were 0.08 (0.07 - 0.09) and 0.16 (0.15 - 0.18) mg/L, respectively.
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