JAUNE REACTIF 181

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 August 1992 to 09 September 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

Deviations:

yes

Remarks:

Mean worm weight is 210 mg based on 60 worms

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Identification Code: FAT 40400/A
Batch No.: BG 3247/TV6
Appearance: red solid
Purity: 84 %
Solubility (in water): ca.100 g/l
Storage: room temperature
Stability: 03/95

Analytical monitoring:

no

Vehicle:

no

Details on preparation and application of test substrate:

- Stock solution: 6 g test substance were mixed with and made up to 500 mL with water.
- Method of mixing into soil: 2.5, 7.4, 22.2, 67 and 200 mL of stock solution were mixed each and made up to 810 mL with water. These solutions were added to the dry premixed artificial soil and homogeneously mixed in a HOBART-blender .

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

- Strain: Earthworm (Eisenia foetida foetida)
- Supplier: Dr. T. Wilde, Farm-Research, D-7867 Wehr/Baden, Germany
- Number of worms: 40 per concentration and control, 4 replicates of 10 worms each.
- Feeding: Included in the soil supplied with the worms and additional feeding during cultivation. No feeding during exposure.
- Age: Adult mature with clitellum (> 2 month of age)
- Adaptation: 24 h conditioning in soil used for the test.
- Keeping: Earthworms arriving from the were kept for 124 days in the original soil before the start 24h-conditioning. Moisture content : approx. 70 % of dry weight of substrate.
- Temperature: 22 ± 2 °C

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Test temperature:

22±2 °C

pH:

6.2-6.5

Moisture:

At the start of exposure: 27.5 - 32.3 % (of dry weight of soil)
At the end of exposure: 24.2 - 31 % (of dry weight of soil)

Details on test conditions:

- Vessels: Beakers of l liter filled with 750 g soil (wet weight), covered with perforated plastic film.
- Soil: According to the guideline
- Temperature: 20 ± 1 °C
- Light: Continuous illumination / 400-800 Lux cold white fluorescent light

Nominal and measured concentrations:

- Nominal concentrations: 12.3, 37, 111, 333 and 1000 mg/kg of dry soil.

Reference substance (positive control):

not specified

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: None

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

> 1 000 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Validity criteria fulfilled:

yes

Conclusions:

LC50 of FAT 40400/A for 7 and 14 days could not be quantified and so determined to be >1000 mg/kg of dry weight of soil.

Executive summary:

The LC50 of the test substance to the earthworm Eisenia foetida was determined in an acute toxicity study performed according to OECD guideline 207 under GLP. In this study 40 earthworms per concentration of test substance were exposed. The concentrations are 12.3, 37, 111, 333 and 1000 mg of test substance per kg of artificial soil d.w. and a control. After 7 and 14 days, no mortality, flaccidity or other abnormalities were observed. Based on these findings the LC50 was determined at >1000 mg/L.

Description of key information

The acute toxicity of the test substance to the earthworm Eisenia foetida was determined in a GLP-compliant study according to OECD guideline 207. In this study, 4 replicates of 10 earthworms were exposed to nominal concentrations of 0 (control), 12.3, 37, 111, 333 and 1000 mg test substance per kg of soil d.w. After 7 and 14 days, mortality, flaccidity and live weights of surviving organisms were recorded. No effects were noted on mortality and flaccidity. Decreased average live weights of test organisms were seen at all test concentrations, but did not significantly differ from controls. Based on these findings, the 14 day LC50 value is determined to be 1000 mg/kg soil (d.w.).

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

1 000 mg/kg soil dw