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EC number: 236-050-7 | CAS number: 13122-18-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-10-23 to 1993-06-29
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1984
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Each test dilution was prepared from an aqueous stock solution (2 L) in which an appropriate weight of test material, mixed with acetone, was added to the dilution water: it was treated by ultrasound (10 minutes) to aid dispersion before use.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Fish Network Limited, Devon
- Length at study initiation (length definition, mean, range and SD): 4.3 cm
- Weight at study initiation (mean and range, SD): 1.0 g
- Feeding during test
- Food type: proprietary trout pellets (BP Nutrition Ltd., Mainstream Trout Fry 02)
- Amount: amount equivalent to approximately between 1 and 4 % of the total wet-weight of the fish in the holding tank.
- Frequency: each day during the holding period - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- c (CaCO3): 244 to 246 mg/L
- Test temperature:
- 14.7 ± 0.6 °C
- pH:
- pH: 7.4 to 8.0
- Nominal and measured concentrations:
- nominal concentrations: 0.75, 1.5, 3, 6, 12 and 24 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: The test vessels were all-glass aquaria, with a total capacity of 15 litres. The depth of the test solutions in these vessels was approximately 14.5 cm. Aeration of the contents of each vessel was achieved using a Pasteur pipette connected to an oil-free supply of compressed air.
TEST MEDIUM / WATER PARAMETERS
Monitoring water quality during the 14-day holding period immediately preceding the definitive test showed the following:
- temperatures ranged from 12.2 to 14.2 °C
- pH values ranged from 7.8 to 8.0
- dissolved oxygen concentrations (DO) ranged from 96 to 100% air saturation value (ASV)
- water hardness ranged from 244 to 250 mg/L as CaCO3
OTHER TEST CONDITIONS
The study was carried out in a temperature-controlled area nominally at 15 ± 2 °C. The day length in the test area was controlled giving a photoperiod of 16 hours light, supplied by overhead fluorescent tubes, and eight hours darkness. Dawn and dusk were simulated by periods of subdued lighting at the beginning and end of each light phase. - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 12%
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 24%
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 6%
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7.03 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL: 12%
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- No positive control
- Reported statistics and error estimates:
- Statistical analysis:
Median lethal concentrations (LC50s) were estimated by the computer program of Stephan using the number of fish exposed and the number dead at each nominal Trigonox 42S concentration. - Sublethal observations / clinical signs:
Cumulative mortality:
Nominal Trigonox 42S concentrations, mg/L
Observation times (hours)
0
0*
0.75
1.5
3
6
12
24
2
0
0
0
0
0
0
0
0
4
0
0
0
0
0
0
2
3
24
0
0
0
0
0
0
3
10
48
0
0
0
0
0
0
3
10
72
0
0
0
0
0
3
10
10
96
0
0
0
0
0
3
10
10
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute lethal toxicity of tert-Butylperoxy-3,5,5-trimethylhexanoat to the rainbow trout was assessed over a period of 96 hours: a semi-static exposure regime was employed with renewal of control and test media after 48 hours.
The highest nominal concentration at which no mortalities occurred and the lowest at which there was 100% mortality after 96 hours was 3 and 12 mg/L respectively.
Treatment-related effects, which were seen at and above 6 mg/l, included darkened pigmentation, lethargic behaviour and loss of coordination. At 12 and 24 mg/L all fish were affected after exposure to the test material for 24 hours and were all dead by 27 and 48 hours respectively. At 6 mg/L, two fish were affected at 24 and 48 hours: at 72 hours three had died and a further three were affected.
The no-observed-effect concentration (NOEC) was therefore 3 mg/L. - Executive summary:
Tert-Butylperoxy-3,5,5-trimethylhexanoat was tested in an acute toxicity test with the rainbow trout under semi-static conditions in accordance with EEC method C.1 (1984) and OECD Test Guideline 203 (1984).
No chemical analysis of control and test media was undertaken.
Groups of ten fish were exposed to tert-Butylperoxy-3,5,5-trimethylhexanoat at nominal concentrations of 0.75, 1.5, 3, 6, 12 and 24 mg/L, selected
following a preliminary, range finding test. Each test dilution was individually prepared by adding the test material to dilution water: acetone and sonication were used to aid dispersion. Control groups of fish were placed in dilution water alone or dilution water containing acetone at the same level as in the test dilutions (0.1 mL/L).
The test was conducted at 14.7 ±0.6°C in treated tap water of hardness 244 to 246 mg/L as CaCO3 at pH values in the range 7.4 to 8.0. The test media were colourless with undissolved material visible on their surfaces; the amount of material increased with increasing concentration.
Observations of the fish were made at least at 24 -hour intervals.
The highest nominal concentration at which no mortalities occurred and the lowest at which there was 100% mortality after 96 hours was 3 and 12 mg/L respectively.
Treatment-related effects, which were seen at and above 6 mg/L, included darkened pigmentation, lethargic behaviour and loss of coordination. At 12 and 24 mg/L all fish were affected after exposure to the test material for 24 hours and were all dead by 27 and 48 hours respectively. At 6 mg/l, two fish were affected at 24 and 48 hours: at 72 hours three had died and a further three were affected.
The no-observed-effect concentration (NOEC) was therefore 3 mg/L.
Median lethal concentrations (LC50s), calculated at intervals during the test using nominal exposure concentrations, are listed below.
Observation intervals (hours)
Approximate LC50s mg/L
24 & 48
14.1 (12 & 24)
72 & 96
7.03 (6 & 12)
( ) : concentration used to calculate the LC50.
Since mortality was progressive during the test, an asymptotic LC50 may not have been attained.
Reference
Description of key information
tert-Butylperoxy-3,5,5-trimethylhexanoat was tested for acute toxicity to fish to EU-method C.1 (1984) and OECD guideline 203 (1984). In this 96-hour semi-static acute toxicity test with rainbow trout, the LC50 value was 7.03 mg/L. The no-observed-effect concentration (NOEC) was 3 mg/L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 7.03 mg/L
Additional information
tert-Butylperoxy-3,5,5-trimethylhexanoat was assessed in short-term toxicity to fish study, according to EU method C.1 and OECD guideline 203.
Fish (rainbow trout) were exposed in a semi-static test to the test item for 96 hours at nominal concentrations of 0.75, 1.5, 3, 6, 12 and 24 mg/L. The LC50 values was 7.03 mg/L. The no-observed-effect concentration (NOEC) was therefore 3 mg/L.
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