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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Acute Immobilization study of test chemical on Daphnia magna in a static System for 48 hrs. Test performed according to the OECD guideline 202.
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
24 and 48 hrs
Hardness:
150.5 mg of CaCO3
Test temperature:
20±2
pH:
7.2
Nominal and measured concentrations:
1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids

OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark

Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Other details not known

Table 1: Immobility

Nominal concentration (mg/l)

No of daphnids

Immobility

 

0 hrs

24 hrs

48 hrs

Control

10

-

-

-

1.562

10

-

-

-

3.125

10

-

-

-

6.25

10

-

-

-

12.5

10

-

-

-

25

10

-

-

-

Any        Abnormal

Behaviour (if any)

 

No effects observed

No effects observed

No effects observed

Table 2: pH and oxygen content:

 

 

 

Nominal

Number of

pH(mg/L)

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

Control

10

7.2

7.3

7.4

 

 

1.562

10

7.3

7.4

7.5

 

 

3.125

10

7.3

7.4

7.6

 

 

6.25

10

7.4

7.5

7.6

 

 

12.5

10

7.4

7.6

7.7

 

 

25

10

7.5

7.6

7.7

 

 

Nominal

Number of

DO(mg/L)

 

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

Control

10

6.8

6.7

6.7

 

 

1.562

10

6.8

6.5

6.5

 

 

3.125

10

6.8

6.5

6.5

 

 

6.25

10

6.8

6.4

6.4

 

 

12.5

10

6.8

6.4

6.4

Test temperature

25

10

6.8

6.4

6.4

Nominal

Number of

Temparature

 

 

 

 

 

 

 

Concentration

daphnids

0h

48h

 

 

 

(mg/l)

 

 

 

 

 

 

Control

10

21.9

 

21.7

 

 

1.562

10

21.9

 

21.7

 

 

3.125

10

21.9

 

21.7

 

 

6.25

10

21.9

 

21.7

 

 

12.5

10

21.9

 

21.7

 

 

25

10

21.9

 

21.7

Validity criteria fulfilled:
yes
Remarks:
1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
Conclusions:
Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l.
Executive summary:

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

Description of key information

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
25 mg/L

Additional information

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

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