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EC number: 500-151-7 | CAS number: 61791-12-6 1 - 6.5 moles ethoxylated
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed according to the guideline.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Principles of method if other than guideline:
- Acute Immobilization study of test chemical on Daphnia magna in a static System for 48 hrs. Test performed according to the OECD guideline 202.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- - Common name: Water flea
- Source: Own breeding of daphnia magna
- Food: No feeding after hatching - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- 24 and 48 hrs
- Hardness:
- 150.5 mg of CaCO3
- Test temperature:
- 20±2
- pH:
- 7.2
- Nominal and measured concentrations:
- 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beaker
- Aeration: No aeration during experiment
- Material, size, headspace, fill volume: 25 ml of glass beaker filled with 20 ml media having headspace of 5 ml
- No. of organisms per vessel: 10 daphnids
OTHER TEST CONDITIONS
- Photoperiod:16 hours light and 8 hours dark
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: Other details not known
- Validity criteria fulfilled:
- yes
- Remarks:
- 1. In the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels
- Conclusions:
- Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l.
- Executive summary:
Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.
Reference
Table 1: Immobility
Nominal concentration (mg/l) |
No of daphnids |
Immobility
|
||
0 hrs |
24 hrs |
48 hrs |
||
Control |
10 |
- |
- |
- |
1.562 |
10 |
- |
- |
- |
3.125 |
10 |
- |
- |
- |
6.25 |
10 |
- |
- |
- |
12.5 |
10 |
- |
- |
- |
25 |
10 |
- |
- |
- |
Any Abnormal Behaviour (if any) |
|
No effects observed |
No effects observed |
No effects observed |
Table 2: pH and oxygen content: |
|
|||||
|
|
Nominal |
Number of |
pH(mg/L) |
|
|
|
|
Concentration |
daphnids |
0h |
48h |
|
|
|
(mg/l) |
|
|
|
|
|
|
Control |
10 |
7.2 |
7.3 |
7.4 |
|
|
1.562 |
10 |
7.3 |
7.4 |
7.5 |
|
|
3.125 |
10 |
7.3 |
7.4 |
7.6 |
|
|
6.25 |
10 |
7.4 |
7.5 |
7.6 |
|
|
12.5 |
10 |
7.4 |
7.6 |
7.7 |
|
|
25 |
10 |
7.5 |
7.6 |
7.7 |
|
|
Nominal |
Number of DO(mg/L) |
|
||
|
|
Concentration |
daphnids |
0h |
48h |
|
|
|
(mg/l) |
|
|
|
|
|
|
Control |
10 |
6.8 |
6.7 |
6.7 |
|
|
1.562 |
10 |
6.8 |
6.5 |
6.5 |
|
|
3.125 |
10 |
6.8 |
6.5 |
6.5 |
|
|
6.25 |
10 |
6.8 |
6.4 |
6.4 |
|
|
12.5 |
10 |
6.8 |
6.4 |
6.4 |
Test temperature |
25 |
10 |
6.8 |
6.4 |
6.4 |
|
Nominal |
Number of Temparature |
|
||||
|
|
|
||||
|
|
Concentration |
daphnids |
0h |
48h |
|
|
|
(mg/l) |
|
|
|
|
|
|
Control |
10 |
21.9 |
|
21.7 |
|
|
1.562 |
10 |
21.9 |
|
21.7 |
|
|
3.125 |
10 |
21.9 |
|
21.7 |
|
|
6.25 |
10 |
21.9 |
|
21.7 |
|
|
12.5 |
10 |
21.9 |
|
21.7 |
|
|
25 |
10 |
21.9 |
|
21.7 |
Description of key information
Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 25 mg/L
Additional information
Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.
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