Castor oil, ethoxylated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Experimental test result performed according to the guideline.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Principles of method if other than guideline:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).

GLP compliance:

no

Analytical monitoring:

no

Remarks:

Test substance is stable under recomended conditions

Vehicle:

no

Details on test solutions:

The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was
found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.8 cm
- Weight at study initiation (mean and range, SD): average 0.17 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Hardness:

150.5 mg of CaCO3

Test temperature:

23 °C

pH:

7.2

Dissolved oxygen:

6.9 mg/l

Nominal and measured concentrations:

0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.

Details on test conditions:

TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 7.33 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: No mortality was observed

Details on results:

Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L.

Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3h		6h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
0.45	4	4	-	-	-	-	-	-	-	-	-	-	-	-
0.91	4	4	-	-	-	-	-	-	-	-	-	-	-	-
1.83	4	4	-	-	-	-	-	-	-	-	-	-	-	-
3.66	4	4	-	-	-	-	-	-	-	-	-	-	-	-
7.33	4	4	-	-	-	-	-	-	-	-	-	-	-	-

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.45

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.91

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

1.83

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

3.66

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

7.33

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

8.2. pH and oxygen content:

		Number of	pH readings after
Nominal	fish		24h		48h		72h		96h
Concentration			V1	V2	V1	V2	V1	V2	V1	V2
(mg/l)
Control	4	4	7.5	7.5	7.7	7.7	7.8	7.8	7.9	7.9
0.45	4	4	7.6	7.6	7.8	7.8	7.9	7.9	8.0	8.0
0.91	4	4	7.6	7.6	7.9	7.9	8.0	8.0	8.1	8.1
1.83	4	4	7.7	7.7	8.0	8.0	8.1	8.1	8.2	8.2
3.66	4	4	7.7	7.7	8.0	8.0	8.1	8.1	8.2	8.2
7.33	4	4	7.7	7.7	8.0	8.0	8.1	8.1	8.2	8.2

 

 

Nominal	Number of		Oxygen content (mg/L) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	7.0	7.0	6.8	6.8	6.7	6.7	6.6	6.6
0.45	4	4	7.0	7.0	6.7	6.7	6.6	6.6	6.5	6.5
0.91	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.5	6.5
1.83	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.5	6.5
3.66	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.4	6.4
7.33	4	4	6.9	6.9	6.7	6.7	6.6	6.6	6.4	6.4

Test temprature:

Nominal	Number of		Temperature (°C) after
Concentrationfish			24h		48h		72h		96h
(mg/l)			V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2
0.45	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2
0.91	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2
1.83	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2
3.66	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2
7.33	4	4	22.9	22.9	24.1	24.1	24.1	24.1	24.2	24.2

Validity criteria fulfilled:

yes

Conclusions:

Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be > 7.33 mg/l.

Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively. and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l . Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

7.33 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.