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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).
GLP compliance:
no
Analytical monitoring:
no
Remarks:
Test substance is stable under recomended conditions
Vehicle:
no
Details on test solutions:
The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was
found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.8 cm
- Weight at study initiation (mean and range, SD): average 0.17 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Hardness:
150.5 mg of CaCO3
Test temperature:
23 °C
pH:
7.2
Dissolved oxygen:
6.9 mg/l
Nominal and measured concentrations:
0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.
Details on test conditions:
TEST SYSTEM
- Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark

Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 7.33 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: No mortality was observed
Details on results:
Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L.
Sublethal observations / clinical signs:

8.1. Mortality and symptoms

Nominal

Concentration

(mg/l)

Number of

fish

Mortality (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.45

4

4

-

-

-

-

-

-

-

-

-

-

-

-

0.91

4

4

-

-

-

-

-

-

-

-

-

-

-

-

1.83

4

4

-

-

-

-

-

-

-

-

-

-

-

-

3.66

4

4

-

-

-

-

-

-

-

-

-

-

-

-

7.33

4

4

-

-

-

-

-

-

-

-

-

-

-

-

Visible Symptoms:

Nominal

Concentration

(mg/l)

Number of

fish

symptom (cumulated) after

3h

6h

24h

48h

72h

96h

 

 

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.45

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

0.91

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

1.83

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

3.66

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

7.33

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

8.2. pH and oxygen content:

 

 

Number of

pH readings after

 

 

 

 

Nominal

fish

24h

 

48h

 

72h

 

96h

 

Concentration

 

 

V1

V2

V1

V2

V1

V2

V1

V2

(mg/l)

 

 

 

 

 

 

 

 

 

 

Control

4

4

7.5

7.5

7.7

7.7

7.8

7.8

7.9

7.9

0.45

4

4

7.6

7.6

7.8

7.8

7.9

7.9

8.0

8.0

0.91

4

4

7.6

7.6

7.9

7.9

8.0

8.0

8.1

8.1

1.83

4

4

7.7

7.7

8.0

8.0

8.1

8.1

8.2

8.2

3.66

4

4

7.7

7.7

8.0

8.0

8.1

8.1

8.2

8.2

7.33

4

4

7.7

7.7

8.0

8.0

8.1

8.1

8.2

8.2

 

 

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

7.0

7.0

6.8

6.8

6.7

6.7

6.6

6.6

0.45

4

4

7.0

7.0

6.7

6.7

6.6

6.6

6.5

6.5

0.91

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

1.83

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.5

6.5

3.66

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.4

6.4

7.33

4

4

6.9

6.9

6.7

6.7

6.6

6.6

6.4

6.4

Test temprature:

 

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

0.45

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

0.91

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

1.83

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

3.66

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

7.33

4

4

22.9

22.9

24.1

24.1

24.1

24.1

24.2

24.2

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be > 7.33 mg/l.
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively. and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l . Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
7.33 mg/L

Additional information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

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