Castor oil, ethoxylated

Endpoint summary

Description of key information

Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L,and 7.33 mg/L,respectively.and Zebra FishDanio reriowere exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration can not be considered for classifying the test material as no mortality were obderved till the last limit of solubility.

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a fresh water system, the NOEC value for the substance is estimated to be1.29e-009mg/l for fish for 28 days of exposure duration. As no effec were observed thus chemical cannot be classify.

Toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected . The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilising agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test  chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a freshwater system, the NOEC value for the test substance is estimated to be1.6e-009mg/l for aquatic invertebrate for 21 days of exposure. As no effect were observed on invertebrate, thus chemical cannot be classify.

Toxicity to aquatic algae and cyanobacteria:

The test chemical  is  likely to be not toxic to aquatic algae and cyanobacteria  in the dose range of 230 mg/l.

Toxicity to microorganism:

The test chemical  is  likely to be toxic to microorganism  in the dose range of  62-220 mg/l .

Additional information

 Short term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was poorly soluble in water. Therefore, the stock solution was prepared by dissolving 5 g of the test substance in 5 liters of potable water (passed through reverse osmosis system) with continuous 72 hrs stirring, after stirring the stock solution was filter and analytically detected and the stock concentration was found to be 7.33 mg/L, by considering this value further calculations did for achieving test concentrations of 0.45 mg/L,0.91 mg/L,1.83 mg/L,3.66 mg/L, and 7.33 mg/L, respectively and Zebra Fish Danio rerio were exposed to these concentration for 96 hours.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to nominal test concentrations, LC50 was determine to be >7.33 mg/l .Test substance was poorly soluble in RO water, In house water solubility for this cas was found to be 24.11 mg/L but in RO water stock concentration found to be 7.33 mg/L after giving 72 hrs stirring, hence by considering this value (7.33 mg/L) further test concentrations were decided and no mortality found till the end of the test hence LC 50 reported was >7.33 mg/L. Hence the above effect concentration cannot be considered for classifying the test material as no mortality were observed till the last limit of solubility.

 

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a fresh water system, the NOEC value for the substance is estimated to be1.29e-009mg/l for fish for 28 days of exposure duration. As no effec were observed thus chemical cannot be classify.

 

Toxicity to aquatic invertebrate:

Daphnia sp., Acute Immobilization test according to OECD Guideline 203 was conducted for test chemical. Test conducted under the static system. The Stability of test substance was determined as per the spectrophotometric method and found to be stable 2 days at 25°C. The test substance was slightly soluble in water. Therefore, the stock solution was prepared by dissolving 100 mg of the test substance in 100 ml of ADaM’s media with continuous 72 hours stirring and this stock was analytically detected. The concentration obtained was then used to prepare the test solution for achieving the test concentrations of 1.5625 mg/L, 3.125 mg/L, 6.25 mg/L, 12.5 mg/L & 25 mg/L, respectively and test Daphnia magna were exposed to these concentration for 48 hours. Test consider to be valid as follows both the criteria. Firstly in the control, including the control containing the solubilizing agent, not more that 10 percent of the daphnids should have been immobilized. 2. The dissolved oxygen concentration at the end of the test should be 3 mg/l in control and test vessels. Based on nominal concentrations, experimental median effective Concentrations [EC-50 (48 h)] for test chemical on test daphnia magna was determine to be > 25 mg/L. As no effect were observed at the highest limit solubility dose 25 mg/l. Thus we cannot classified chemical as toxic and in cannot consider in any classified criteria.

 

Long term toxicity to aquatic invertebrate:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on aquatic invertebrate was predicted for test substance . On the basis of effects observed in a freshwater system, the NOEC value for the test substance is estimated to be1.6e-009mg/l for aquatic invertebrate for 21 days of exposure. As no effect were observed on invertebrate, thus chemical cannot be classify.

 

Toxicity to aquatic algae and cyanobacteria:

Data available for the structurally and functionally similar read across chemicals has been reviewed to determine the toxicity of aquatic algae and cyanobacteria of the test chemical .The studies are as mentioned below:

 

To study the effects of test material, ethoxylated toxicity to aquatic algae and cyanobacteria test was carried out for 72 hr. Effective concentration EC50 to 50% of Desmodesmus subspicatus when exposed to test material for 72 hr is 6.61 mg/L.

 

First study was supported by the second. Test material was used to evaluate its toxicity on Chlorococcales green algae for 24 hrs in static condition. The effect concentration (EC50) at which population of algae was effect was observed to be 230 mg/l. The test chemical is not likely to be toxic to aquatic algae and cyanobacteria in the dose range of 23 mg/l.

 

Based on the above effect concentrations it can be concluded that test material is toxic to aquatic algae and can be classified as aquatic chronic 3 as per CLP criteria.

 

Toxicity to microorganism:

Data available for the test chemical and read across chemical selected on the basis of structural similarity has been reviewed to determine the toxicity of microorganism of the test chemical. The studies are as mentioned below:

 

In the first study Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxicity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 220 mg/L.

 

Similarly cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxicity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Based on the above all studies and data for aquatic fish and invertebrates, test chemical cannot be considered as toxic as no mortality, mobility and growth inhibition were observed till the last limit of solubility.