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EC number: 629-776-4 | CAS number: 308065-15-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Aug - 28 Sep 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Biodegrability test
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- mixture of sewage, soil and natural water
- Details on inoculum:
- - Source of inoculum/activated sludge (location, sampling depth): location: 10 sites (4 sewage plants, 3 rivers, 1 lake and 2 bays) around Japan, sampling depth: return sludge in sewage plants, surface water and surface soil of foreshore
- Laboratory culture: Thirty minutes after stopping of aeration, discard about one third of the whole volume of supernatant and add an equal volume of a solution (pH adapted to 7.0 with NaOH) containing 0.1% each of glucose, peptone and pottasium orthophosphate, to the settled material and re-commence aeration. This procedure was repeated once per day.
- Temperature: 25 ± 2 °C
- Concentration of sludge: 30 mg/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: recommended medium in the guideline (JIS K 0102-1993)
- Test temperature: 25 ± 1 °C
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: 300 mL culture bottle
- Number of culture flasks/concentration: 1 (water + test substance), 3 (sludge + test sub.), 1 (sludge + reference sub.), 1 (sludge)
- Measuring equipment: Coulometer
- Test performed in closed vessels due to significant volatility of test substance: Yes
- Details of trap for CO2 and volatile organics if used: soda lime
SAMPLING
- Sampling frequency: 7, 14, 21, 28 days
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Toxicity control: no
- Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 78
- Sampling time:
- 28 d
- Details on results:
- No 10-day window required. Substance was readily biodegradable.
Partial hydrolisation of the test substance in water to form dodecanoic acid and methanol was described. - Results with reference substance:
- The oxygen consumption for aniline in the above used test system reached 62% and 75% degradation after 7 days and 14 days.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- yes
- Remarks:
- adaptations for poorly soluble substances made (BODIS method)
- GLP compliance:
- no
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Sewage treatment plant Hochdahl, municipal sewage, serving a population equivalent of 27000
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 100 other: mg
- Based on:
- ThOD/L
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Concentration of elements in test medium (according to OECD 301 Guideline)
P 116 mg/L
N 1.3 mg/L
Na 86 mg/L
K 122 mg/L
Mg 2.2 mg/L
Ca 9.9 mg/L
Fe 0.01-0.05 mg/L
- Mineral test medium was inoculated with 2.5-3.5 mL activated sludge, stirred and stabilised for one week. Then, it was distributed at 200 to 300 mL into test bottles, bottles were aerated with compressed air until O2 saturation was reached and then spiked with a pre-determined amount of test material
- Test temperature: 20-25 °C, however since the O2 measurements should be carried out at 20 ± 0.5 °C, the temperature was adjusted half an hour before
TEST SYSTEM
- Culturing apparatus: Bottles with sintered glass tube head
- Number of culture flasks/concentration: 3
- Method used to create aerobic conditions: Compressed air
- Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 83
- Sampling time:
- 28 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 68
- Sampling time:
- 7 d
- Details on results:
- The test substance reached 83% degradation on day 28, fulfilling the 10-d window criterion. Therefore, it can be considered readily biodegradable
- Results with reference substance:
- Sodium acetate reached 93% biodegradation within 14 days and a final biodegradation value of 94% on day 28
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Category justification is attached in chapter 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 83
- Sampling time:
- 28 d
- Remarks on result:
- other: CAS 6788-59-8
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 78
- Sampling time:
- 28 d
- Remarks on result:
- other: CAS 111-82-0
Referenceopen allclose all
Table 1. Table of BOD
|
7thday |
14thday |
21stday |
28thday |
|
||||
|
BOD (mg) |
Deg(%) |
BOD (mg) |
Deg(%) |
BOD (mg) |
Deg(%) |
BOD (mg) |
Deg(%) |
MeanDeg. % |
Sludge+Aniline |
57.4 |
62 |
71.0 |
75 |
71.5 |
75 |
75.0 |
76 |
|
Water + Testsub. |
0 |
- |
0.2 |
- |
0.2 |
- |
0.2 |
- |
|
Controlblank |
1.6 |
- |
3.4 |
- |
3.7 |
- |
6.7 |
- |
|
Sludge+ Testsub. |
40.5 |
46 |
57.1 |
64 |
68.3 |
77 |
73.1 |
79 |
78 |
Sludge+ Testsub. |
31.3 |
35 |
48.2 |
53 |
60.1 |
67 |
68.3 |
73 |
|
Sludge+ Testsub. |
32.3 |
37 |
51.7 |
58 |
65.5 |
74 |
75.3 |
82 |
|
Deg. =( BOD- control blank) / TOD x 100 TOD of test substance: 83.9 mg TOD of aniline: 90.3 mg
Table 2.Result of analysis for BOD and GC
|
water+ testsub. |
sludge+ testsub. (*1) |
TheoreticalAmount |
|||
sampleNo. 2 |
sampleNo. 4 |
sampleNo. 5 |
sampleNo. 6 |
|||
BOD |
mg |
0.2 |
66.4 |
61.6 |
68.6 |
83.9 |
GC (residue of test substance) |
mg |
26.9 |
0.0 |
0.0 |
0.0 |
30.4 |
% (*2) |
89 |
0 |
0 |
0 |
- |
|
GC (production ofdodecanoicacid) |
mg |
1.1 |
0.0 |
0.0 |
0.0 |
28.4 |
% (*2) |
4 |
0 |
0 |
0 |
- |
(*1) (sludge+ test sub.)was calculated by subtracting blank values.
(*2) Residue and production percentage was calculated as followed.
Residue percentage = Residue amount (mg) /Theoretical amount (mg) x 100
Production percentage = Production amount (mg) /Theoretical amount (mg) x 100
Table 1. % Biodegradation measured as O2 consumption of test and reference substance
Day |
TS |
Ref substance |
7 |
67 |
86 |
14 |
81 |
93 |
21 |
82 |
94 |
28 |
83 |
94 |
Description of key information
Readily biodegradable:
> 70% in 28 days (read across)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
As no experimental data evaluating the potential for ready biodegradability of Fatty acids, C12-C14 (even numbered), methyl esters (CAS No. 308065-15-8) are available, in accordance to Regulation (EC) No. 1907/2006, Annex XI, 1.5, read-across from structurally related category members, methyl laurate (CAS No. 111-82-0) and Fatty acids, coco, Me esters (CAS No. 61788-59-8) is performed. All three substances are characterized as esters of methanol and either C12 to C14 fatty acids (CAS No. 308065-15-8 and CAS No. 61788-59-8) or only C12 fatty acids (CAS No. 111-82-0). Therefore, their biodegradation potential is expected to be comparable and the read-across is considered justified.
The study evaluating the biodegradation potential of methyl laurate (CAS No. 111-82-0, Japanese Ministry of Economy, Trade and Industry, 1995) was conducted according to OECD 301C (Modified MITI Test), under GLP conditions. A mixture of sewage, soil and natural water was used as inoculum and O2 consumption monitored as indicator of biodegradation. After 28 days of incubation, the test substance reached 78% biodegradation. Since the 10-day window concept does not apply to the MITI method, methyl laurate can be considered readily biodegradable according to OECD criteria. On the other hand, the test investigating the potential for ready biodegradability of Fatty acids, coco, Me esters (CAS No. 61788-59-8, Richterich, 2001) was performed following a method equivalent to OECD Guideline 301D, adapted for poorly soluble substances (BODIS test). This substance reached 83% biodegradation after 28 days, fulfilling the 10-day window criterion (> 60% biodegradation within this period) and therefore it is readily biodegradable too.
Based on the results obtained for the structurally related category members (in accordance to Regulation (EC) No. 1907/2006, Annex XI, 1.5), Fatty acids, C12-C14 (even numbered), methyl esters can be considered as readily biodegradable.
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