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Tert.-Butylperoxy-2-ethylhexanoat was tested for aquatic toxicity in a number of tests including EU method C.1, Acute toxicity to fish, EU method C.2, Acute toxicity to Daphnia and EU method C.3, Acute toxicity to algae. Following results were obtained:

Acute toxicity to fish:

An acute test following EU method C.1 with rainbow trout revealed a 96-h LC50 of 8.66 mg/L (nominal concentration). The 96-h NOEC was 2.10 mg/L (nominal concentration).

Acute toxicity to aquatic invertebrates:

An acute test following EU method C.2 with Daphnia magna revealed a 48-h EC50 of 7.5 mg/L (nominal concentration).

Acute toxicity to algae:

An acute test following EU method C.3 with Pseudokirchneriella subcapitata revealed a 72-h EC50 (growth rate) of 0.4394 mg/L.

The 72-h NOEC (growth rate) was 0.018 mg/L.

Long-term toxicity to daphnia:

A long-term toxicity to daphnia test following OECD Guideline 211 with Daphnia magna revealed a 21-d NOEC value of 0.45 mg/L, LOEC value of 0.87 mg/L and EC50 value of 1.02 mg/L (based on reproduction and measured concentrations).

Toxicity to microorganisms:

Tert.-Butylperoxy-2-ethylhexanoat was assessed in an activated sludge following EU method C.11 with microorganisms. The 30 min EC50 was determined to be 64 mg/L (nominal concentration).

Long-term toxicity testing to fish is waived according to REACH Annex IX Sect. 9.1 Col 2.