Registration Dossier
Registration Dossier
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EC number: 203-403-1 | CAS number: 106-49-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
Data source
Reference
- Title:
- No information
- Author:
- Nalecz-Jawecki G, Sawicki J (1999). SPIROTOX - A new tool for testing the toxicity of volatile compounds. Chemosphere 38 (14), 3211-3218.
Materials and methods
- Principles of method if other than guideline:
- Spirotox test: Toxicity of volatile compounds to the protozoa Spirostomum ambiguum was tested in polysterene multiwell plates.
- GLP compliance:
- no
Test material
- Reference substance name:
- p-toluidine
- EC Number:
- 203-403-1
- EC Name:
- p-toluidine
- Cas Number:
- 106-49-0
- Molecular formula:
- C7H9N
- IUPAC Name:
- 4-methylaniline
- Details on test material:
- - Name of test material (as cited in study report): p-toluidine
- Analytical purity: analytical purity
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- - stock dilutions were prepared in deionized water or, if a toxicant was insoluble in water, in methanol
- dilutions were prepared using Tyrod solution (see ¿any other information on materials..." => "Test organism")
Test organisms
- Test organisms (species):
- other: protozoa: Spirostomum ambiguum
Test conditions
- Details on test conditions:
- Type: aquatic
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.8 mmol/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: SD ± 0.41
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.93 mmol/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: SD ± 0.01
- Duration:
- 24 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 2.87 mmol/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: SD ± 0.55
- Duration:
- 48 h
- Dose descriptor:
- other: LC50
- Effect conc.:
- 1.52 mmol/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: SD ± 0.26
Any other information on results incl. tables
All results are expressed in mmol/l. The 24-h values correspond to:
=> 24-h EC50 = 192.9 mg/l
=> 24-h LC50 = 307.5 mg/
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
