Phosphoryl trichloride

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Older guideline-comparable study reported in summary form

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1978

Report date:

1978

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

other: Class B poison test

GLP compliance:

no

Remarks:

: study pre-dates GLP

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phosphoryl trichloride

EC Number:

233-046-7

EC Name:

Phosphoryl trichloride

Cas Number:

10025-87-3

Molecular formula:

Cl3OP

IUPAC Name:

phosphoroyl trichloride

Test animals

Species:

rabbit

Strain:

New Zealand White

Sex:

male/female

Details on test animals or test system and environmental conditions:

Five rabbits (three male and two female) New Zealand albino rabbits in the weight range of between 1.9 kg and 2.5 kg were used in this experiment.

Administration / exposure

Type of coverage:

not specified

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

No details specified

Duration of exposure:

24 hour exposure

Doses:

Four dose levels were used in this study: 398, 631, 1000 and 1580 mg/kg bw.

No. of animals per sex per dose:

Three male and two females

Control animals:

no

Details on study design:

One male rabbit was administered with the 398 mg/kg bw dose, one female rabbit received the 631 mg/kg bw dose, one male and one female rabbit received the 1000 mg/kg bw dose and one male rabbit received the 1580 mg/kg bw dose.

Statistics:

No additional information

Results and discussion

Preliminary study:

None

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

1 000 mg/kg bw

Remarks on result:

other: 1/2 deaths at this dose level

Mortality:

No deaths were recorded at dose levels of 398 and 631 mg/kg bw, where as one death (1/2) was recorded at the 1000 mg/kg bw level and one death was recorded at the 1580 mg/kg bw level

Clinical signs:

other: Signs of toxicity were recorded as follows: weight loss (two to three days in survivors), increasing weakness, collapse and death

Gross pathology:

Lung and liver hyperaemia, enlarged gall bladder, kidney discolouration in one instance and gastrointestinal inflammation. After 14 days the viscera of the survivors appeared normal

Other findings:

No additional information

Any other information on results incl. tables

No deaths were recorded at dose levels of 398 and 631 mg/kg bw, where as one death (1/2) was recorded at the 1000 mg/kg bw level by day 4 and one death was recorded at the 1580 mg/kg bw level by day 2 after dosing. Signs of systemic toxicity (weight loss (in survivors), increasing weakness, collapse and death) were observed.

Dose level mg/kg bw	Average initial weight		Mortalities / Dosed			Time of Mortality
	Male	Female	Male	Female	Combined
398	2.5	-	0/1	-	0/1	-
631	-	2.0	-	0/1	0/1	-
1000	2.1	2.0	0/1	1/1	1/2	4 days
1580	1.9	-	1/1	-	1/1	2 days

Applicant's summary and conclusion

Conclusions:

Based on the mortality of 1/2 animals at this dose level, the acute dermal LD50 value is estimated to be 1000 mg/kg bw.

Executive summary:

Phosphoryl trichloride was applied (occlusion and duration not specified) to five rabbits (three male and two female) at dose levels of 398 (1), 631 (1), 1000 (2) and 1580 mg/kg bw (1). Deaths occurred at the 1000 mg/kg bw (1/2) by day four after dosing, and 1580 mg/kg bw (1/1) by day 2 after dosing.

Based on the results of this study, the acute dermal LD50 of phosphoryl trichloride is estimated to be 1000 mg/kg bw.