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EC number: 282-015-4 | CAS number: 84082-70-2 Extractives and their physically modified derivatives such as tinctures, concretes, absolutes, essential oils, oleoresins, terpenes, terpene-free fractions, distillates, residues, etc., obtained from Mentha piperita, Labiatae.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- weight of evidence
- Study period:
- 31-Aug-2010
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Test was conducted according to OECD Test Guideline No. 437, 2009, under GLP Standards, and QA. Due to the read-across purpose it was given a Klimisch 2 rating, in accordance with the ECHA Practical guide #6 on the reporting of read-across in IUCLID. The justification for read across is provided in the attached background material of the chapter summary.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guideline for Testing of Chemicals 437: Bovine Corneal Opacity and Permeability (BCOP) Test Method for Identifying Ocular Corrosives and Severe Irritants (September, 2009)
- Deviations:
- no
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Mentha arvensis, ext.
- EC Number:
- 290-058-5
- EC Name:
- Mentha arvensis, ext.
- Cas Number:
- 90063-97-1
- IUPAC Name:
- 90063-97-1
- Reference substance name:
- Cornmint oil
- IUPAC Name:
- Cornmint oil
- Details on test material:
- - Name of test material (as cited in study report): Cornmint oil (mentha arvensis)
- Substance type: Clear colourless liquid
- Physical state: Liquid
- Composition of test material, percentage of components: Confidential
- Lot/batch No.: Confidential
- Stability under test conditions: Stable
- Storage condition of test material: In refrigerator (2-8°C) protected from light
Constituent 1
Constituent 2
Test animals / tissue source
- Species:
- other: not relevant: in vitro test
- Strain:
- other: Bovine
- Details on test animals or tissues and environmental conditions:
- BOVINE EYES:
- Source: Bovine eyes from young cattle were obtained from the slaughterhouse, where the eyes were excised by a slaughterhouse employee as soon as possible after slaughter.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent vehicle
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 750 ul per cornea
NEGATIVE CONTROL
- Amount(s) applied (volume or weight with unit): 750 ul of physiological saline per cornea
POSITIVE CONTROL
- Amount(s) applied (volume or weight with unit): 750 ul per cornea
- Concentration (if solution): 10% (w/v) Benzalkonium Chloride - Duration of treatment / exposure:
- Exposure: 10 minutes
Post incubation period: 120 minutes - Observation period (in vivo):
- Not applicable
- Details on study design:
- TEST SITE: Isolated bovine cornea
REMOVAL OF TEST SUBSTANCE
- Washing (if done): yes, 3 times
- Time after start of exposure: 10 minutes
SCORING SYSTEM:
- After the 10 minutes exposure and washing step, the corneas were incubated for 120 +/- 10 minutes at 32 °C. After the completion of the incubation period opacity determination was performed by sodium fluorecein treatment. Incubation 90 min. Optical density of the medium determined at 490 nm.
- The mean opacity and mean permeability values (OD490) were used for each treatment group to calculate an in vitro score:
In vitro irritancy score (IVIS) = mean opacity value + (15 x mean OD490 value).
TOOL USED TO ASSESS SCORE: opacitymeter and microplate reader.
DATA EVALUATION: A test substance that induces an IVIS ≥ 55.1 is defined as a corrosive or severe irritant.
Results and discussion
In vivo
Results
- Irritation parameter:
- other: In vitro irritancy score (IVIS)
- Basis:
- mean
- Time point:
- other: 10 minutes
- Score:
- ca. 4.3
- Reversibility:
- other: not applicable
- Other effects:
- No pH effect of the test substance was observed on the rinsing medium.
Applicant's summary and conclusion
- Interpretation of results:
- other: not severe irritant or corrosive
- Remarks:
- Criteria used for interpretation of results: EU
- Conclusions:
- In the in vitro Bovine Corneal Opacity and Permeability test, cornmint oil (mentha arvensis) did not induce ocular irritation through both endpoints, opacity and permeability, resulting in a mean in vitro irritancy score of 4.3 after 10 minutes of treatment. It is concluded that this test is valid and that Cornmint oil (mentha arvensis) is not severe irritant or corrosive in the BCOP test under the experimental conditions described in the report.
- Executive summary:
This in vitro study was performed according to OECD Guideline 437 (2009) to assess the corneal irritation and damage potential of Cornmint oil (mentha arvensis) by means of the Bovine Corneal Opacity and Permeability Assay (BCOP) using fresh isolated bovine corneae. The negative control responses of the opacity and permeability values were less than the upper limits of the laboratory historical range indicating that the negative control did not induce irritancy on the corneas. The mean in vitro irritancy score of the positive control (10% (w/v) Benzalkonium Chloride) was 119 and was within the historical positive control data range. It was therefore concluded that the test conditions were adequate and that the test system functioned properly. Cornmint oil (mentha arvensis) did not induce ocular irritation through both endpoints, opacity and permeability, resulting in a mean in vitro irritancy score of 4.3 after 10 minutes of treatment. Finally, it is concluded that this test is valid and that Cornmint oil (mentha arvensis) is not severe irritant or corrosive in the BCOP test under the experimental conditions described in the report.
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