4,4'-oxydi(benzenesulphonohydrazide)

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Details on test solutions:

Stock solution was prepared by diluting test chemical in dimethylformamide (200,000 mg/L). Test solutions were prepared by adding the corresponding amounts of stock solution into 1,000 mL of OECD medium. The concentration of solvent was 100 µL/L.
OECD M4 medium was applied.
Test concentrations were measured 3 times during 21 days before and after renewal of test solutions

Test organisms (species):

Daphnia magna

Details on test organisms:

Age: Juveniles within 24 hrs old
Supplier: Independent Administrative Institution National Institute of Environmental Studies, Japan
Daphnids were fed Chlorella vulgaris for 0.15 mg C /day/animal during the test periods.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Post exposure observation period:

no

Hardness:

250 mg/L as CaCO3

Test temperature:

20 ± 1 °C

pH:

7.5 ~ 8.8

Dissolved oxygen:

7.3 ~ 8.6 mg/L (over 60 % of the air saturation value)

Nominal and measured concentrations:

Nominal
Con.
(mg/L) Measured Con. (mg/L) TWM** (mg/L) % of Nominal
0 day (new) 1 day (old) 7 day (new) 8 day (old) 14 day (new) 15 day (old)
mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*) mg/L (%*)
Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
Solvent Control <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) <0.002 (–) – –
0.400 0.370 (93) 0.155 (39) 0.335 (84) 0.143 (36) 0.327 (82) 0.129 (32) 0.229 57
0.850 0.779 (92) 0.332 (39) 0.706 (83) 0.298 (35) 0.760 (89) 0.288 (34) 0.495 58
1.80 1.59 (88) 0.684 (38) 1.68 (93) 0.633 (35) 1.60 (89) 0.674 (37) 1.07 59
3.80 3.32 (87) 1.33 (35) 3.28 (86) 1.32 (35) 3.09 (81) 1.29 (34) 2.13 56
8.00 7.05 (88) 2.74 (34) 6.94 (87) 2.69 (34) 6.53 (82) 2.72 (34) 4.47 56

Details on test conditions:

Light: 800 Lux, Light periodicity: 16/8 (light/dark)

Reference substance (positive control):

not specified

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

2.13 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

4.47 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

2.48 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LC50

Effect conc.:

2.13 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

21day-EC50 (95 % confidence limits) of OBSH to Daphnia magna was 2.48 mg/L (2.31 ~ 3.63 mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

The LC50 value and the 95 % confidence limits were calculated by Probit Analysis or Binominal or Moving average method. The EC50 value and the 95 % confidence limits were calculated by Logistic regression analysis method. The NOEC and the LOEC value were analysed by Bartlett test and 1-way ANOVA or Kruskal-Wallis test.

21day-EC₅₀(95 % confidence limits) of OBSH toDaphnia magnawas 2.48 mg/L (2.31~3.63mg/L). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.

Validity criteria fulfilled:

not specified

Conclusions:

21 d-NOEC (reproduction) = 2.13 mg/L (TWA)

Executive summary:

The chronic toxicity of OBSH towards Daphnia magna was assessed applying the OECD Guidline No. 211.

The following concentrations were tested: Control, Solvent control, 0.400, 0.850, 1.80, 3.80 and 8.00 mg/L.

Based on the results, a 21day-NOEC (reproduction) of OBSH to Daphnia magna was determined to be 2.13 mg/L (TWA, semi-static). At the end of the test, the mortality of the parent daphnids was 0 % and 10 % in the control and solvent control, respectively. The mean number of live offspring produced per parent animal surviving at the end of the test was 92 animals and 107 animals in control and solvent control, respectively.

Description of key information

A reliable long-term toxicity study (21 d, semi-static) to Daphnia magna was carried out according to OECD 211, resulting in a 21 d-NOEC (reproduction) of 2.13 mg/L (TWA).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

2.1 mg/L

Additional information

A 21 -d reproduction study on Dapnhia magna showed an NOEC of 2.1 mg/L (Guideline unknown).