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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data
Analytical monitoring:
yes
Details on sampling:
Test concentrations were measured at the beginning (0 hr) of the study and 24 hrs after exposure (before renewal of test solution)
Details on test solutions:
Stock solution was prepared by diluting test chemical in dimethylformamide (200,000 mg/L). Test solutions were prepared by adding the corresponding amounts of stock solution into 500 mL of OECD M4 medium. The concentration of solvent was 100 µL/L.
Nominal concentration: Control, solvent control, 2.0, 3.6, 6.0, 11.2, 20.0 mg/L
Number of Replicate: 4
Daphnia per test concentration: 5
Test solutions were renewed after 24 hrs.
Test organisms (species):
Daphnia magna
Details on test organisms:
Age: Juveniles within 24 hrs old
Supplier: Independent Administrative Institution National Institute of Environmental Studies, Japan

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Test concentrations were measured at the beginning (0 hr) of the study and 24 hrs after exposure (before renewal of test solution) using HPLC.
Post exposure observation period:
no
Hardness:
250 mg/L as CaCO3
Test temperature:
20 ± 1 °C
pH:
7.7 ~ 8.4
Dissolved oxygen:
8.0 ~ 8.8 mg/L (over 60 % of the air saturation value)
Nominal and measured concentrations:
Nominal Con. (mg/L) Measured Con. (mg/L) Geometric Mean Conc. During 24 hrs (mg/L)
0 hr (New*) % of Nominal 24 hrs (Old**) % of Nominal
Control < 0.02 – < 0.02 – –
Solvent Control < 0.02 – < 0.02 – –
2.00 1.73 87 0.78 39 1.16
3.60 2.93 81 1.24 34 1.91
6.40 5.29 83 2.17 34 3.39
11.20 9.12 81 3.80 34 5.89
20.0 16.2 81 6.50 33 10.3


Details on test conditions:
Dilution water source: OECD M4 medium (Hardness: 250 mg/L as CaCO3)
Water chemistry: DO = 8.0 ~ 8.8 mg/L (over 60 % of the air saturation value), pH = 7.7 ~ 8.4
Temperature: 20 ± 1 °C
Light: 800 Lux, Light periodicity: 16/8 (light/dark)
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.9 mg/L
Nominal / measured:
meas. (geom. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 2.9 mg/L (2.44 ~ 3.47 mg/L). No immobilisation and adverse effects were observed in the control and solvent control.
No immobilisation and adverse effects were observed in the control. At 24 hrs, 14 and 26 daphnids were immobilized with 20 and 40 mg/L of OBSH, respectively. At 48 hrs, 6, 20, and 30 daphnids were immobilized with 10, 20, and 40 mg/L of OBSH. Any abnormal behaviors of the daphnids was not observed in each treatment group during the testing.
Precipitation of the test substance and formation of film at the surface were observed in more than 1 mg/L but immobilisation was observed in more than 10 mg/L. Therefore immobilization of daphnia and film formation are not related to each other.
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
Data Analysis: The EC50 value (α=0.05) was calculated by Probit Analysis method.

Measured concentration of the test substance in test water under the semi-static condition

Nominal Con. (mg/L)

Measured Con. (mg/L)

Geometric Mean Conc.

During 24 hrs (mg/L)

0 hr (New*)

% of Nominal

24 hrs (Old**)

% of Nominal

Control

< 0.02

< 0.02

Solvent Control

< 0.02

< 0.02

2.00

1.73

87

0.78

39

1.16

3.60

2.93

81

1.24

34

1.91

6.40

5.29

83

2.17

34

3.39

11.20

9.12

81

3.80

34

5.89

20.0

16.2

81

6.50

33

10.3

Results of acute toxicity test to Daphnia magna   (Unit mg/L)                                                           

Exposure time

24 hrs

48 hrs

EC50*

9.8

2.9

95% confidence limits

8.3~13.4

2.4~3.5

Highest test concentration resulting in 0% mortality

3.4

< 1.2

Lowest test concentration resulting in 100% mortality

> 10.3

10.3

The results of cumulative immobilization data for Daphnia magna

Nominal Conc.(mg/L)

Mean Measured Conc.(mg/L)a

Number of

Organisms tested

Cumulative Number of

Organisms Immobilized (%immobility)

24 hrs

48 hrs

Control

20

0

0

Solvent Control

20

0

0

2.00

1.16

20

0

1 (5)

3.60

1.91

20

0

3 (15)

6.40

3.39

20

0

12 (60)

11.20

5.89

20

2 (10)

19 (95)

20.0

10.3

20

11 (55)

20 (100)

Validity criteria fulfilled:
not specified
Conclusions:
48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 2.9 mg/L (2.44 ~ 3.47 mg/L). No immobilisation and adverse effects were observed in the control and solvent control.
Executive summary:

The acute toxicity of OBSH towards Daphnia magna was tested accoring to the OECD Guideline no. 202. The study was conducted according to GLP. Juvenile daphnids (> 24h) were exposed for 48 h under static conditions. The following concentrations were applied: Control,solvent control, 2.0, 3.6, 6.0, 11.2, 20.0mg/L. Based on the results the 48 hr-EC50 (α=0.05) of OBSH to Daphnia magna was 2.9 mg/L (2.44 ~ 3.47 mg/L). No immobilisation and adverse effects were observed in the control and solvent control during the study.

Description of key information

A reliable short-term (48 hr, semi-static) toxicity study to aquatic invertebrates was carried out according to OECD 202, resulting in an 48h-EC50 of 2.9 mg/L (mean measured).


The findings are supported in another study (static test condistions) with following results: 48h-EC50 (immobility) = 0.69 mg/L (mean measured).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Remarks:
mobility
Effect concentration:
2.9 mg/L

Additional information