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Administrative data

Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Qualifier:
according to guideline
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes
Type of method:
HPLC estimation method
Media:
soil/sewage sludge

Test material

Constituent 1
Chemical structure
Reference substance name:
Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)
EC Number:
404-310-0
EC Name:
Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)
Cas Number:
10591-85-2
Molecular formula:
C30H28N2S4
IUPAC Name:
N,N-dibenzyl[(dibenzylcarbamothioyl)disulfanyl]carbothioamide
Details on test material:
- Name of test material (as cited in study report): Linkwell TBzTD

- Substance type: mono-constituent substance
- Physical state: solid, powder
- Analytical purity: > 99%

- Lot/batch No.: 08060902
- Expiration date of the lot/batch: June 2009
- Storage condition of test material: room temperature
Radiolabelling:
no

Study design

Test temperature:
ambient (25 °C)

HPLC method

Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC System HP 1100 (Agilent)
- Type, material and dimension of analytical (guard) column: Zorbax CN, 250 x 4 mm, 5 µm
- Detection system: refractometer HP 1100 (Agilent)

MOBILE PHASES
- Type: acetonitrile/water (purified) 60/40 (v/v)
- Experiments with additives carried out on separate columns: no
- Flow rate: 1.0 ml/min
- Injection volume: 20 µl
- Temperature: ambient (25 °C)


DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column: urea as inert marker

REFERENCE SUBSTANCES
- Identity: Acetanilide, Monuron, Linuron; Atrazine, Fenthion, Trifluralin

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: see Table 1
- Quantity of reference substances: see Table 1


REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': k'=(tR-t0)/t0
- Determination of the log Koc value:
Extrapolated from linearized function log Koc = f(log k' ) of the calibration data
(extrapolation was necessary because in the elution area of the test item no calibration substances with good correlation were available).

Results and discussion

Adsorption coefficient
Type:
log Koc
Value:
4.8
Temp.:
25 °C

Results: HPLC method

Details on results (HPLC method):
- Retention times of reference substances used for calibration: see Table 2
- Details of fitted regression line (log k' vs. log Koc): correlation coefficient r = 0.9814
- Graph of regression line attached: yes
- Average retention data for test substance: 9.7 min

further details see Table 2

Applicant's summary and conclusion

Validity criteria fulfilled:
yes