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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 2011-11-25 to 2012-01-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study was performed under GLP and according to OECD guideline
Reason / purpose for cross-reference:
reference to same study
Qualifier:
according to guideline
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Type of method:
HPLC estimation method
Media:
soil/sewage sludge
Radiolabelling:
no
Test temperature:
30° C (column temperature)
Details on study design: HPLC method:
EQUIPMENT
- Apparatus: HPLC
- Type: Agilent Technologies 1200 Series, incorporating autosampler
- Type, material and dimension of analytical (guard) column: Luna 5µ CN 100A (150 x 4.6 mm id)
- Detection system: UV

MOBILE PHASES
- Type: methano1:water 55:45 v/v
- pH: 5.99
- Solutes for dissolving test and reference substances: methanol

DETERMINATION OF DEAD TIME
- Method: by inert substances which are not retained by the column

REFERENCE SUBSTANCES
- Identity: acetanilide, phenol, atrazine, isoproturon, triadimenol, linuron, naphthalene, endosulfan-diol, fenthion, α-endosulfan, phenanthrene, diclofop-methyl, DDT

DETERMINATION OF RETENTION TIMES
- Quantity of test substance introduced in the column: 1 µg (injection volume: 10 µL, sample solution conc: 0.1 g/L)
- Quantity of reference substances:
acetanilide: 1.16 µg
phenol: 1.13 µg
atrazine: 1.03 µg
isoproturon: 1.14 µg
triadimenol: 1.19 µg
linuron: 1.13 µg
naphthalene: 1.05 µg
endosulfan-diol: 1.50 µg
fenthion: 1.26 µg
α-endosulfan: 1.33 µg
phenanthrene: 1.20 µg
diclofop-methyl: 1.29 µg
DDT: 1.60 µg
- Intervals of calibration: not reported

REPETITIONS
- Number of determinations: 2

EVALUATION
- Calculation of capacity factors k': (retention time substance - dead time)/dead time
- Determination of the log Koc value: by linear regression of log k' values versus log Koc
Duration:
30 min
Initial conc. measured:
0.1 other: g/L
pH:
5.99
Temp.:
30 °C
Type:
log Koc
Value:
3.61
Temp.:
30 °C
Type:
Koc
Value:
4 070
Temp.:
30 °C
Details on results (HPLC method):
- Retention times of reference substances used for calibration:
acetanilide: 2.417 mins
phenol: 2.376 mins
atrazine: 2.675 mins
isoproturon: 2.874 mins
triadimenol: 3.034 mins
linuron: 3.319 mins
naphthalene: 4.038 mins
endosulfan-diol: 3.338 mins
fenthion: 4.738 mins
α-endosulfan: 5.544 mins
phenanthrene: 6.256 mins
diclofop-methyl: 5.814 mins
DDT: 13.056 mins
- Details of fitted regression line (log k' vs. log Koc): slope = 0.334; y-axis intercept = -1.0912, r = 0.9631
- Graph of regression line attached: yes
- Average retention data for test substance: 4.674 mins
Validity criteria fulfilled:
not applicable
Conclusions:
The adsorption coefficient of the test item has been determined to be Koc = 4070 or log Koc = 3.61.
Executive summary:

The adsorption coefficient was determined according to OECD TG 121 to be 4.07 x l0^3, log Koc 3.61, using the HPLC screening method.

Description of key information

The adsorption coefficient Koc was determined via HPLC estimation method to be 4070.

Key value for chemical safety assessment

Koc at 20 °C:
4 070

Additional information

The adsorption coefficient was determined according to OECD TG 121 to be 4.07 x l0^3, log Koc 3.61, using the HPLC screening method.

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