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EC number: 205-381-9 | CAS number: 139-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- The study with the read across substance is considered sufficient to fulfil the information requirements.
- Reason / purpose for cross-reference:
- read-across source
- Principles of method if other than guideline:
- seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Species:
- Phaseolus vulgaris
- Plant group:
- Dicotyledonae (dicots)
- Test type:
- seedling emergence toxicity test
- Study type:
- laboratory study
- Substrate type:
- other: seedlings grown in polystyrene squares
- Limit test:
- no
- Total exposure duration:
- 14 d
- Key result
- Species:
- Phaseolus vulgaris
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 58.4 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- seedling emergence
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In a 14 day xeposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
- Executive summary:
In a 14 day exposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
- Endpoint:
- toxicity to terrestrial plants: long-term
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- The study with the read across substance is considered sufficient to fulfil the information requirements.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- no data available
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:
pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 % - Species:
- other: Nicotiana tabacum
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Tobacco plants (N. tabacum SR-1) were used for the experiment.
- Test type:
- vegetative vigour test
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 21 d
- Remarks:
- approximately 3 weeks
- Post exposure observation period:
- no data available
- Test temperature:
- 25/20 °C light/dark temperature regime
- pH:
- 6.40 - 6.83
- Moisture:
- 60% relative humidity
- Details on test conditions:
- 16 h light period (light intensity of 320 µmol/m2 * s)
- Nominal and measured concentrations:
- nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
- Reference substance (positive control):
- not specified
- Key result
- Species:
- other: Nicotiana tabacum
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.25 - < 1.25 other: mmol/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Remarks on result:
- other: equivalent to > 84- <420 mg/kg soil dw
- Details on results:
- EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
- Results with reference substance (positive control):
- no data available
- Reported statistics and error estimates:
- no data availble
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
- Executive summary:
The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.
According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.
The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
- Long-term EC10, LC10 or NOEC for terrestrial plants:
- 86 mg/kg soil dw
Additional information
There are no studies available on the toxicity of trisodium HEDTA to terrestrial plants, however the effects of disodium EDTA and EDTA were investigated in tobacco (Nicotiana tabacum) and bean (Phaseolus vulgaris), respectively. Na2-EDTA had an inhibitory effect on the growth ofNicotiana tabacumat a rate of <1.25 mmol/kg soil dw (equivalent to <420 mg/kg soil dw). The NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil dw). A 14 day exposure to EDTA showed no inhibitory effect on seedling emergence and growth of bean plants up to a concentration of 58.4 mg/kg soil. The 14 d NOEC of 84 mg Na2-EDTA/kg soil dw was retained for the CSA. Assuming a purity of 100% for the test substance, this is equivalent to ca. 86 mg Na3-HEDTA (based on a molecular weight of 336.21 for Na2-EDTA and 344.20 for Na3-HEDTA).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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