Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)

Toxicity to terrestrial plants

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The study with the read across substance is considered sufficient to fulfil the information requirements.

Reason / purpose for cross-reference:

read-across source

Principles of method if other than guideline:

seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Species:

Phaseolus vulgaris

Plant group:

Dicotyledonae (dicots)

Test type:

seedling emergence toxicity test

Study type:

laboratory study

Substrate type:

other: seedlings grown in polystyrene squares

Limit test:

no

Total exposure duration:

14 d

Key result

Species:

Phaseolus vulgaris

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

> 58.4 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

seedling emergence

Validity criteria fulfilled:

not specified

Conclusions:

In a 14 day xeposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.

Executive summary:

In a 14 day exposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.

Reference 2

Endpoint:

toxicity to terrestrial plants: long-term

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The study with the read across substance is considered sufficient to fulfil the information requirements.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

no data available

Vehicle:

not specified

Details on preparation and application of test substrate:

A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:

pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 %

Species:

other: Nicotiana tabacum

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

Tobacco plants (N. tabacum SR-1) were used for the experiment.

Test type:

vegetative vigour test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

21 d

Remarks:

approximately 3 weeks

Post exposure observation period:

no data available

Test temperature:

25/20 °C light/dark temperature regime

pH:

6.40 - 6.83

Moisture:

60% relative humidity

Details on test conditions:

16 h light period (light intensity of 320 µmol/m2 * s)

Nominal and measured concentrations:

nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA

Reference substance (positive control):

not specified

Key result

Species:

other: Nicotiana tabacum

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

> 0.25 - < 1.25 other: mmol/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other: equivalent to > 84- <420 mg/kg soil dw

Details on results:

EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.

Results with reference substance (positive control):

no data available

Reported statistics and error estimates:

no data availble

Validity criteria fulfilled:

not specified

Conclusions:

Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).

Executive summary:

The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.

According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.

The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:

86 mg/kg soil dw

Additional information

There are no studies available on the toxicity of trisodium HEDTA to terrestrial plants, however the effects of disodium EDTA and EDTA were investigated in tobacco (Nicotiana tabacum) and bean (Phaseolus vulgaris), respectively. Na2-EDTA had an inhibitory effect on the growth ofNicotiana tabacumat a rate of <1.25 mmol/kg soil dw (equivalent to <420 mg/kg soil dw). The NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil dw). A 14 day exposure to EDTA showed no inhibitory effect on seedling emergence and growth of bean plants up to a concentration of 58.4 mg/kg soil. The 14 d NOEC of 84 mg Na2-EDTA/kg soil dw was retained for the CSA. Assuming a purity of 100% for the test substance, this is equivalent to ca. 86 mg Na3-HEDTA (based on a molecular weight of 336.21 for Na2-EDTA and 344.20 for Na3-HEDTA).