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Toxicity to terrestrial plants

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Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
supporting study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The study with the read across substance is considered sufficient to fulfil the information requirements.
Reason / purpose:
read-across source
Principles of method if other than guideline:
seedlings of Phaseolus vulgaris were exposed to EDTA for 14 days
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Species:
Phaseolus vulgaris
Plant group:
Dicotyledonae (dicots)
Test type:
seedling emergence toxicity test
Study type:
laboratory study
Substrate type:
other: seedlings grown in polystyrene squares
Limit test:
no
Total exposure duration:
14 d
Key result
Species:
Phaseolus vulgaris
Duration:
14 d
Dose descriptor:
NOEC
Effect conc.:
> 58.4 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Validity criteria fulfilled:
not specified
Conclusions:
In a 14 day xeposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.
Executive summary:

In a 14 day exposure test EDTA showed no inhibitory effect on seedling emergence and growth of Phaseolus vulgaris up to a concentration of 58.4 mg/kg soil.

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
no data available
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The study with the read across substance is considered sufficient to fulfil the information requirements.
Reason / purpose:
read-across source
Qualifier:
according to
Guideline:
OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
GLP compliance:
not specified
Analytical monitoring:
not specified
Details on sampling:
no data available
Vehicle:
not specified
Details on preparation and application of test substrate:
A silty–loamy sand agricultural soil, USDA (Soil Survey Manual), was collected from 0 to 30 cm surface layer of the Melaten field in Aachen, Germany. The soil was air-dried at room temperature, sieved through a 2-mm sieve and characterised as follows:

pH: 6.8
organic matter: 3.5 %
soil fractions
sand: 49.4 %
Clay: 42.1 %
Silt: 8.5 %
Species:
other: Nicotiana tabacum
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
Tobacco plants (N. tabacum SR-1) were used for the experiment.
Test type:
vegetative vigour test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
21 d
Remarks:
approximately 3 weeks
Post exposure observation period:
no data available
Test temperature:
25/20 °C light/dark temperature regime
pH:
6.40 - 6.83
Moisture:
60% relative humidity
Details on test conditions:
16 h light period (light intensity of 320 µmol/m2 * s)
Nominal and measured concentrations:
nominal: 0.125,0.25, 1.25, 62.5, 125, and 250 mmol/kg for Na2EDTA
Reference substance (positive control):
not specified
Key result
Species:
other: Nicotiana tabacum
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
> 0.25 - < 1.25 other: mmol/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks on result:
other: equivalent to > 84- <420 mg/kg soil dw
Details on results:
EDTA did not show any adverse effects at a concentration of < 0.125 mmol/kg. At 1.25 mmol/kg of EDTA the leaves showed necrotic lesions and the dry weight was negligible. At a concentration of 0.25 mmol/kg EDTA the dry weight is only slightly lower but the standard deviation is higher in comparison to the 0.125 mmol/kg application.
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
no data availble
Validity criteria fulfilled:
not specified
Conclusions:
Based on the results of this vegetative vigour study EDTA had an inhibitory effect on growth of Nicotiana tabacum at rate of 1.25 mmol/kg soil (equivalent to 420 mg/kg). The NOEC will be determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil).
Executive summary:

The effects of EDTA (Na2EDTA) on vegetative vigour of plants following application were examined in a laboratory test on Nicotiana tabacum. The test item was applied at a dose rate of up to 250 mmol/kg and compared to an untreated control.

According to the results, the test item had an inhibitory effect on growth of the tested species at rates of 1.25 mmol/kg soil (equivalent to 420 mg/kg) and thus determined to be the overall LOEC for this species.

The overall NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg).

Description of key information

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for terrestrial plants:
86 mg/kg soil dw

Additional information

There are no studies available on the toxicity of trisodium HEDTA to terrestrial plants, however the effects of disodium EDTA and EDTA were investigated in tobacco (Nicotiana tabacum) and bean (Phaseolus vulgaris), respectively. Na2-EDTA had an inhibitory effect on the growth ofNicotiana tabacumat a rate of <1.25 mmol/kg soil dw (equivalent to <420 mg/kg soil dw). The NOEC was determined to be 0.25 mmol/kg soil (equivalent to 84 mg/kg soil dw). A 14 day exposure to EDTA showed no inhibitory effect on seedling emergence and growth of bean plants up to a concentration of 58.4 mg/kg soil. The 14 d NOEC of 84 mg Na2-EDTA/kg soil dw was retained for the CSA. Assuming a purity of 100% for the test substance, this is equivalent to ca. 86 mg Na3-HEDTA (based on a molecular weight of 336.21 for Na2-EDTA and 344.20 for Na3-HEDTA).