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EC number: 205-381-9 | CAS number: 139-89-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- The study with the read across substance is considered sufficient to fulfil the information requirements.
- Reason / purpose for cross-reference:
- read-across source
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- not specified
- Remarks:
- Published data, GLP status not indicated
- Analytical monitoring:
- not specified
- Details on sampling:
- no data
- Vehicle:
- no
- Details on preparation and application of test substrate:
- EDTA was applied in deionized water solution into artificial soil (35 % dry weight moisture content).
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- no data available
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Post exposure observation period:
- no data available
- Test temperature:
- 20 °C
- pH:
- no data available
- Moisture:
- 35 % of maximum holding capacity
- Details on test conditions:
- Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.
- Nominal and measured concentrations:
- 0, 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/KG dry weight soil.
- Reference substance (positive control):
- not specified
- Key result
- Duration:
- 14 d
- Dose descriptor:
- EC50
- Effect conc.:
- 156.46 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 136.01 - 179.98
- Results with reference substance (positive control):
- no data available
- Reported statistics and error estimates:
- Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests. - Validity criteria fulfilled:
- not specified
- Conclusions:
- The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.
- Executive summary:
The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.
Reference
Description of key information
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 184 mg/kg soil dw
Additional information
There are no studies available on the toxicity of trisodium HEDTA to soil macroorganisms, however the effects of EDTA were investigated in Eisenia fetida. After 14 days of exposure, an LC50 of 156 mg EDTA/kg soil dw was obtained. Assuming 100% purity of the test substance, this is equivalent to ca. 184 mg Na3-HEDTA (based on a molecular weight of 292.24 for EDTA and 344.20 for Na3-HEDTA). This value was retained for the CSA.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

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