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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Link to relevant study record(s)

toxicity to soil macroorganisms except arthropods: short-term
Type of information:
read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
no data available
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
The study with the read across substance is considered sufficient to fulfil the information requirements.
Reason / purpose for cross-reference:
read-across source
according to guideline
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
GLP compliance:
not specified
Published data, GLP status not indicated
Analytical monitoring:
not specified
Details on sampling:
no data
Details on preparation and application of test substrate:
EDTA was applied in deionized water solution into artificial soil (35 % dry weight moisture content).
Test organisms (species):
Eisenia fetida
Animal group:
Details on test organisms:
no data available
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
Total exposure duration:
14 d
Post exposure observation period:
no data available
Test temperature:
20 °C
no data available
35 % of maximum holding capacity
Details on test conditions:
Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.
Nominal and measured concentrations:
0, 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/KG dry weight soil.
Reference substance (positive control):
not specified
Key result
14 d
Dose descriptor:
Effect conc.:
156.46 mg/kg soil dw
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
Remarks on result:
other: 136.01 - 179.98
Results with reference substance (positive control):
no data available
Reported statistics and error estimates:
Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests.
Validity criteria fulfilled:
not specified
The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.
Executive summary:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.

Description of key information

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
184 mg/kg soil dw

Additional information

There are no studies available on the toxicity of trisodium HEDTA to soil macroorganisms, however the effects of EDTA were investigated in Eisenia fetida. After 14 days of exposure, an LC50 of 156 mg EDTA/kg soil dw was obtained. Assuming 100% purity of the test substance, this is equivalent to ca. 184 mg Na3-HEDTA (based on a molecular weight of 292.24 for EDTA and 344.20 for Na3-HEDTA). This value was retained for the CSA.