Trisodium 2-(carboxylatomethyl(2-hydroxyethyl)amino)ethyliminodi(acetate)

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

The study with the read across substance is considered sufficient to fulfil the information requirements.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

not specified

Remarks:

Published data, GLP status not indicated

Analytical monitoring:

not specified

Details on sampling:

no data

Vehicle:

no

Details on preparation and application of test substrate:

EDTA was applied in deionized water solution into artificial soil (35 % dry weight moisture content).

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

no data available

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

no data available

Test temperature:

20 °C

pH:

no data available

Moisture:

35 % of maximum holding capacity

Details on test conditions:

Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.

Nominal and measured concentrations:

0, 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/KG dry weight soil.

Reference substance (positive control):

not specified

Key result

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

156.46 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 136.01 - 179.98

Results with reference substance (positive control):

no data available

Reported statistics and error estimates:

Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests.

Validity criteria fulfilled:

not specified

Conclusions:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.

Executive summary:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was estimated to be 156 mg/kg dry weight soil.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

184 mg/kg soil dw

Additional information

There are no studies available on the toxicity of trisodium HEDTA to soil macroorganisms, however the effects of EDTA were investigated in Eisenia fetida. After 14 days of exposure, an LC50 of 156 mg EDTA/kg soil dw was obtained. Assuming 100% purity of the test substance, this is equivalent to ca. 184 mg Na3-HEDTA (based on a molecular weight of 292.24 for EDTA and 344.20 for Na3-HEDTA). This value was retained for the CSA.