Hydrocortisone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

other information

Study period:

Sep to Oct 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: well reported GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Hydrocortisone

EC Number:

200-020-1

EC Name:

Hydrocortisone

Cas Number:

50-23-7

Molecular formula:

C21H30O5

IUPAC Name:

11,17,21-trihydroxypregn-4-ene-3,20-dione

Details on test material:

- Name of test material (as cited in study report): hydrocortisone (ZK 5191)
- Analytical purity: 99.3%
- Lot/batch No.: 024H0502
- Expiration date of the lot/batch: March 1996

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

One immobilised Daphnia was observed in the control, three in the test solution of 50 mg/L and six in the 100 mg/L test solution at the end of the exposure period.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50/48 hours was higher than 100 mg/L, the NOEC was 25 mg/L the LOEC 50 mg/L.

Executive summary:

The aquatic toxicity of hydrocortison was studied in an acute immobilization test in Daphnia magna. The daphnids were exposed to the test compound at 3 concentrations between 25 and 100 mg/L, measured by TOC, and a control over a period of 48 hours. There was a concentration related immobilization rate up to 30% at the highest concentration. Therefore, the EC50/48 hours was higher than 100 mg/L, the NOEC was 25 mg/L the LOEC 50 mg/L.