Reaction mass of 2,2'-(Ethylenedioxy)diethanol and 3,6,9-Trioxaundecane-1,11-diol

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

other: Am. Publ. Health Assoc. (APHA): Standard Methods for the Examination of Water and Wastewater, 15th ed.

Principles of method if other than guideline:

Method: special acute fish toxicity test system by the Center for Lake Superior Environmental Studies, University of Wisconsin-Superior

GLP compliance:

no

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source (i.e. manufacturer or supplier) and lot/batch number of test material: Eastrnan Kodak Co., Lot no.: C4H1003
- Purity, including information on contaminants, isomers, etc.: 99%

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all chambers at time 0 h, 24 h, 48 h, 72 h, and 96 h. All compound analyses included one spike and one duplicate sample for
every 6 to 12 water samples
- Sampling method: sampled at mid-depth of exposure chamber
- Sample storage conditions before analysis: samples analysed immediately or adequately preserved for later analysis

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Controls: negative control (water only)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Test concentration separation factor: ca. 1.55
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Age at study initiation (mean and range, SD): 30 days

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

45.0 (1.83) mg/L CaCO3

Test temperature:

22.2 (0.41) °C

pH:

7.3 (0.53)

Dissolved oxygen:

6.4 (0.66)

Nominal and measured concentrations:

Measured (corrected average mean): 16.3, 29.0, 54.1, 64.3, 92.3 g/L;
recovery: 102.1% (SD 3.8)

Details on test conditions:

OTHER TEST CONDITIONS
- Adjustment of pH: The pH of the stock solution was adjusted to that of lake water with HCl.
- Exposure volume: 7 L
- 3.4 times exposure volume exchange per day

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass tanks, 7 L
- Aeration: no information
- Type of flow-through (e.g. peristaltic or proportional diluter): proportional diluter
- Renewal rate of test solution (frequency/flow rate): 3.4 v/d
- No. of organisms per vessel: 25
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- Biomass loading rate: 0.05 g/L per 24 h

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: natural water from Lake Superior (data from 1972) or (for few tests) dechlorinated water from City of Superior (WI)
- Total organic carbon: no data
- Metals (µg/L):
- Al: 1-26 / 0.60-1.9
- Cd: < 0.1 / < 0.1
- Cr: 2-20 / < 0.1
- Co: < 0.5 / < 0.5
- Cu: 0.3-3.2/ 2.0-6.0
- Fe: 2-83 / 160-194
- Pb: 7-20 / < 0.1
- Mn: 0.2-11.5 / 6.6-7.8
- Ni: < 0.5 / < 1.0-1.3
- Zn: 1.0-2.7 / 2.2-3.0
- Chloride: 1.2-1.3 mg/L / 3.6-4.1 mg/L
- Alkalinity: 43.1 mg/L as CaCO3
- Ca/Mg ratio: ca. 6.5:1
- Conductivity: 78-86 µmhos/cm / 91-138 µmhos/cm


OTHER TEST CONDITIONS
- Photoperiod: 16 h light : 8 h dark
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- visible abnormalities and mortality: after 0, 2, 3, 6, 12, 24, 48, 72, and 96 h
- pH: at least once in control, low medium and high test concentration
- dissolved oxygen: after 0, 24, and 96 h in control, low medium and high test concentration
- temperature: daily
- analytical quantification of test concentrations: all chambers after 0, 24, 48, 72, and 96 h


TEST CONCENTRATIONS
- Spacing factor for test concentrations: ca. 1.5

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

70 200 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

Validity criteria

• In the control(s) (dilution water control, solvent control), the mortality should not exceed 10% (or one fish, if fewer than 10 control fish are tested) at the end of the exposure: No control fish died during th exposure.
• The dissolved oxygen concentration should be ≥60% of the air saturation value in all test vessels throughout the exposure: The air saturation value was ca. 90%.
• Analytical measurement of test concentrations is compulsory. The test concentrations were stable over the exposure period.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Principles of method if other than guideline:

Method: static fish test

GLP compliance:

no

Specific details on test material used for the study:

- Name of test substance (as cited in publication): Triethylene glycol

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Source: Test organisms obtained from commercial hatcheries from the New York metropolitan area
- Acclimation period: Fish were held for acclimation period of 14 days prior to testing
- Food type: Fish were fed with minced frozen shrimp
- Length at study initiation: 33 - 75 mm
- Fish were not fed 48 h prior to testing
- Fish that were in contact with unhealthy fish were treated for disease prevention immediately before acclimation

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

- Concentrations (ppm): 10000, 7800 (nominal)

Details on test conditions:

TEST SYSTEM
- Test vessels: all-glass, 30 gallon fish aquaria equipped with aeration and charcoal filters
- Dissolved oxygen readings taken daily and pH-value was noted at end of assay time period
- Mortality counts taken daily

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

1. SOFTWARE
Estimation Programs Interface (EPI) Suite for Microsoft Windows, v4.11 (US EPA, 2012)

2. MODEL (incl. version number)
ECOSAR v2.00

3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
See section 'Test Material'.

4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
see attached QSAR report in "Attached justification"

5. APPLICABILITY DOMAIN
see attached QSAR report in "Attached justification"

6. ADEQUACY OF THE RESULT
- The model is scientifically valid (see attached QSAR report in "Attached justification").
- The model estimates the toxicity to aquatic organisms: Acute toxicity to fish (LC50); this data can be used to support the assessment regarding the toxicity of a substance to
aquatic organisms (see attached QSAR report in "Attached justification").

Qualifier:

no guideline followed

Principles of method if other than guideline:

ECOSAR v2.00: Estimation of acute toxicity to fish for chemical class "Neutral Organic SAR (Baseline Toxicity)"

GLP compliance:

no

Test organisms (species):

other: fish

Water media type:

freshwater

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

286 839 mg/L

Nominal / measured:

estimated

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: ECOSAR chemical class: "Neutral Organic SAR (Baseline Toxicity)"; The substance is in the applicability domain of the model.

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

(Q)SAR

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification

Justification for type of information:

1. SOFTWARE
Estimation Programs Interface (EPI) Suite for Microsoft Windows, v4.11 (US EPA, 2012)

2. MODEL (incl. version number)
ECOSAR v2.00

3. SMILES OR OTHER IDENTIFIERS USED AS INPUT FOR THE MODEL
See section 'Test Material'.

4. SCIENTIFIC VALIDITY OF THE (Q)SAR MODEL
see attached QSAR report in "Attached justification"

5. APPLICABILITY DOMAIN
see attached QSAR report in "Attached justification"

6. ADEQUACY OF THE RESULT
- The model is scientifically valid (see attached QSAR report in "Attached justification").
- The model estimates the toxicity to aquatic organisms: Acute toxicity to fish (LC50); this data can be used to support the assessment regarding the toxicity of a substance to
aquatic organisms (see attached QSAR report in "Attached justification").

Qualifier:

no guideline followed

Principles of method if other than guideline:

ECOSAR v2.00: Estimation of acute toxicity to fish for chemical class "Neutral Organic SAR (Baseline Toxicity)"

GLP compliance:

no

Test organisms (species):

other: fish

Water media type:

freshwater

Total exposure duration:

96 h

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

654 324 mg/L

Nominal / measured:

estimated

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Remarks on result:

other: ECOSAR chemical class: "Neutral Organic SAR (Baseline Toxicity)"; The substance is in the applicability domain of the model.

Description of key information

Overall, it can be concluded that based on the available experimental and estimated data for the main constituents of EC 907-131-0, it can be concluded that the substance is with high probability acutely not harmful to fish.

Additional information

QSAR-disclaimer

In Article 13 of Regulation (EC) No 1907/2006, it is laid down that information on intrinsic properties of substances may be generated by means other than tests, provided that the conditions set out in Annex XI (of the same Regulation) are met.

According to Annex XI of Regulation (EC) No 1907/2006 (Q)SAR results can be used if (1) the scientific validity of the (Q)SAR model has been established, (2) the substance falls within the applicability domain of the (Q)SAR model, (3) the results are adequate for the purpose of classification and labeling and/or risk assessment and (4) adequate and reliable documentation of the applied method is provided.

For the assessment of the substance (Q)SAR results were used for the estimation of the acute toxicity to fish. The criteria listed in Annex XI of Regulation (EC) No 1907/2006 are considered to be adequately fulfilled and therefore the endpoint(s) sufficiently covered and suitable for risk assessment.

Assessment

2,2'-(ethylenedioxy)diethanol 3,6,9-trioxaundecane-1,11-diol (EC 907-131-0) is a multi-constituent substance. The main constituents of EC 907-131-0 are triethylene glycol (TGE, CAS 112-27-6, 10-80% w/w) and tetraethylene glycol (Tetra-EG, CAS 112-60-7, 10-80% w/w). Since no data on the short-term toxicity to fish of the reaction mass of 2,2'-(ethylenedioxy)diethanol 3,6,9-trioxaundecane-1,11-diol are available, the assessment is based on the available data for the main components triethylene glycol (CAS 112-27-6) and tetraethylene glycol (112-60-7).

A short-term study using Pimephales promelas was performed with triethylene glycol (CAS 111-27-6). The 96 -h LC50 value was measured to be 70200 mg/L (measured, Geiger, 1988). Additionally, the short-term toxicity test performed with L. macrochirus is available (Dawson et al., 1975). In this study, groups of unknown quantity freshwater fish (Lepomis macrochirus) were exposed for 96 hours to nominal test concentrations of 7800 and 10000 mg/L under static conditions. Analytical verification of the test concentrations was not performed (not specified). The 96-h LC50 was determined to be > 10000 mg/L.

These results are supported with the estimated values, which were derived from a valid QSAR model (ECOSAR v2.0; BASF SE, 2022). The 96 -h LC50 for CAS 112-27-6 was predicted to be 2868390mg/L, while the 96-h LC50 for CAS 112-60-7 was estimated to be 654324 mg/L. Both substances are within the applicability domain of the model.

Overall, it can be concluded that based on the available experimental and estimated data for the main constituents of EC 907-131-0, it can be concluded that the substance is with high probability acutely not harmful to fish.