Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
bacterial growth of Pseudomonas putida
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Principles of method if other than guideline:
Method: other: Bringmann and Kühn (1977), Z. Wasser Abwasser Forsch. 10,
87-98
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- Stock solution: 5.00 g test substance/l (were made up with bidistilled water ); gave pH = 11.5, which was neutralized with 10 % hydrochloric acid.
-Test concentrations ( 500 mg/L; 1000 mg/L ; 2000 mg/L and 4000 mg/L) were prepared by diluting the stock solution with bidestilled water.
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
18 h
Remarks on exposure duration:
+/- 1h
Test temperature:
25 +/- 2 degree C
pH:
7.1 (Test 1); 7.0 (Test 2)
Nominal and measured concentrations:
Nominal: control / 500 / 1,000 / 2,000 / 4,000 mg/l
Details on test conditions:
- Number of replicates: 5 growth controls 4 controls 3 replicates of 4 concentrations all performed twice
- Test vessel: 250 ml Erlenmeyer flasks, sterile, capped with cellulose
- Duration of test: 18 +/- 1 hours
- Quantification: photometric determination of turbidity at 436 nm, graphical evaluation
Reference substance (positive control):
not specified
Key result
Duration:
18 h
Dose descriptor:
EC10
Effect conc.:
1 120 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: extinction/absorption

EC10 = 1200 mg/l (Test 1)
1040 mg/l (Test 2);
mean = 1120 mg/l

Validity criteria fulfilled:
not specified
Conclusions:
The EC10 (18 hours) was determined as 1120 mg/L indicating that the test substance does not pose a significant hazard to aquatic microorganism
Executive summary:

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1,120 mg/l was obtained. Four concentrations ranging from 500 to 4000 mg/L were tested. The study was assessed as "reliable with restrictions".

Description of key information

In a bacterial toxicity test using Pseudomonas putida (Hüls AG, 1996b) an 18 h-EC10 of 1120 mg/l was obtained.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
1 120 mg/L

Additional information

18 h-EC10 value of 1120 mg/l