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Diss Factsheets

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study
Qualifier:
according to guideline
Guideline:
other: OECD 202, part 2 (1984)
Deviations:
not specified
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
MONITORING OF TEST SUBSTANCE CONCENTRATION:
only at >= 1 mg/l; 0.1 and 0.3 mg/l below detection limit
Vehicle:
no
Details on test solutions:
STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dilution watert: M4 medium (Elendt)
- Concentration of stock solution: 0.2 g/l

DILUTION WATER
- Synthetic freshwater; M4-medium according to Elendt (1990)
Test organisms (species):
Daphnia magna
Details on test organisms:
- Strain: Daphnia magna Straus, clone 5
- Supplier: inhouse
- Breeding method: in synthetic medium according to Elendt (1990), water renewal every 2-3 days, isolation of juveniles for further breeding every ca. 4 weeks
- Age/size/weight/loading: < 24 hours
- Feeding: Scenedesmus subspicatus, as much as consumed
- Pretreatment: Filtration of adults 24 h prior to testing
- Feeding during test: Scenedesmus subspicatus, days 0-6: 4.0E+06 cells/(individual x day) days 7-21: 2.0E+07 cells/(individual x day)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d
Test temperature:
20 +/- 1 degree C
pH:
Range of 9 determinations:
control: 7.5-8.0; 0.1 mg/l: 7.1-7.9; 0.3 mg/l: 7.5-7.9; 1.0 mg/l: 7.3-7.9; 3.0 mg/l: 7.6-7.9 ; 10.0 mg/l: 7.6-8.2; 30.0 mg/l: 8.3/8.2 (interrupted after 2 determinations)
Dissolved oxygen:
Range of 9 determinations:
Control: 94-107 %; 0.1 mg/l: 94-105 % ; 0.3 mg/l: 93-100 % ; 1.0 mg/l: 93-105 %; 3.0 mg/l: 95-106 %; 10.0 mg/l: 96-110 %; 30.0 mg/l: 103/113 % (interrupted after 2 determinations)
Nominal and measured concentrations:
Nominal: 0.1; 0.3; 1.0; 3.0; 10.0; 30.0 mg/l
Measured (mean of initical conc.):
Details on test conditions:
- Exposure vessel type: beakers, 250 ml filled with 200 ml test solution (days 0-6), 150 ml filled with 80 ml (days 7-21)
- Number of replicates, individuals per replicate: days 0-6: 4 replicates, 5 individuals each; days 7-21: <= 10 replicates, 1 individual each
- Renewal of test solution: each Monday, Wednesday and Friday
- Intensity of irradiation: 58 W = ca. 1000 Lux
- Photoperiod: 20 hours bright / 4 hours dark
Reference substance (positive control):
not specified
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction

- Concentration / response curve:





















































concentration% mortality% reproduction% inhibition
control051.3+/-13.2-
0.1 mg/l1044.4+/-5.113.5 (not significant)
0.3 mg/l053.1+/-16.4-3.5
1.0 mg/l048.0+/-4.76.4 (not significant)
3.0 mg/l055.7+/-19.6-8.6
10.0 mg/l2042.1+/-14.817.9 (not significant)
30.0 mg/l1000.0+/-0.0100
Validity criteria fulfilled:
not specified
Conclusions:
The long-term NOEC for reproduction (21 days) was determined as 3 mg/L indicating that the test item may have chronic effects on aquatic invertebrates.
Executive summary:

The long-term toxicity of 3-aminomethyl-3,5,5-trimethylcyclohexylamine on Daphnia magna was investigated over a test period of 21 days according to OECD 202, part 2 (1984). The reproduction rate and the mortality of the parent animals were monitored at nominal concentrations ranging from 0.1 to 30.0 mg/L. The long-term NOEC for reproduction (21 days) was determined as 3 mg/L and the LOEC (reproduction) as 10 mg/L.

The study was assessed as "reliable without restrictions".

Description of key information

Partly cited from SIAR for SIAM 18 (Paris, France, April 20–23, 2004):
"The effects of 3-aminomethyl-3,5,5-trimethylcyclohexylamine on the reproduction rate of Daphnia magna were tested in a chronic test according to OECD 202, part 2, but modified according to EC requirements (Hüls AG, 1993c). Under semistatic conditions, the daphnids were exposed for 21
days to concentrations ranging from 0.1 – 30.0 mg/L. Concentrations up to 3.0 mg/l (NOEC) had no influence on survival of the daphnids or their reproduction rate. At 10 mg/l (LOEC), survival was reduced to 80 % with no significant reduction of the reproduction rate. The next (highest) concentration of 30.0 mg/l led to 100 % mortality."

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
NOEC
Effect concentration:
3 mg/L

Additional information

21-d NOEC-value for Daphnia magna.