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EC number: 202-429-0 | CAS number: 95-53-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Link to relevant study record(s)
Description of key information
o-Toluidine is rapidly absorbed via gastrointestinal tract and is rapidly distributed, metabolized and excreted mainly via urine. although there are no special toxicokinetik data on absorption via skin and respiratary tract , absorptions via this administration routes is shown by data from acute toxicity.
Key value for chemical safety assessment
Additional information
OECD/SIDS of o-toluidine published by UNEP in 2006 summarizes:
Studies in Animals:
There are no special toxicokinetic data on absorption via skin and respiratory tract but respective acute toxicity studies indicate absorption via these administration routes.
Following oral application of 500 mg toluidine/kg bw to rats a half-life time of plasma elimination of 12 to 15 hours was derived (Brock, Hundley and Lieder, 1990); i.v. application of app. 111 mg/kg bw to dogs yielded a half-life time of plasma elimination of half an hour (Kiese, 1963). 48 hours following subcutaneous injection of labelled o-toluidine hydrochloride into rats, Son, Everett and Fiala (1980) detected radioactivity in decreasing range: liver > kidney > spleen, colon > lung and bladder. In another study 72 hours following oral application to rats, radioactivity was detected in decreasing range: whole blood > spleen > kidney > liver > subcutaneous abdominal fat > lung > heart > abdominal skin> bladder >gastrointestinal tract > bone marrow > brain > muscle > testes (Brock, Hundley and Lieder, 1990). The results from metabolism study in rats show that N-acetylation and hydroxylation at the position 4 of the aromatic ring of o-toluidine are the major metabolic pathways in rats. Minor pathways include hydroxylation at position 6, oxidation of the methyl-group and oxidation of the amino-group. Sulphate conjugates predominate over glucuronides by a ratio of 6:1. The metabolites which were found included azoxytoluene, onitrosotoluene, N-acetyl-o-toluidine, N-acetyl-o-aminobenzyl alcohol, 4-amino-m-cresol, N-acetyl- 4-amino-m-cresol, anthranilic acid, N-acetyl-anthranilic acid, 2-amino-m-cresol, other unidentified substances and unchanged o-toluidine (Son, Everett and Fiala, 1980).
In rats, unchanged o-toluidine and the metabolites are largely excreted in the urine: 48 hours after s.c. injection up to 83 % of the dose (Son, Everett and Fiala, 1980) and 72 hours after oral application up to 94.7 % of the dose (Cheever, Richards and Plotnick, 1980). Faecal excretion and exhalation of the substance is minimal with up to 3.5 % and 1.46 % of the dose (Son, Everett and Fiala, 1980).
Studies in humans
Biological Monitoring to assess human exposure to o-oluidine indicates that absorption may occur though inhalation and dermal contact; however, quantitative information was not identified. o-toluidine binds to hemoglobin. N-acetylated metabolites of o-toluidine are eliminated in the urine (CICAD 1988)
Overall conclusion
o-Toluidine is rapidly absorbed via gastrointestinal tract and is rapidly distributed, metabolized and excreted mainly via urine. Although there are no special toxicokinetic data on absorption via skin and respiratory tract, absorption via these administration routes is shown by data from acute toxicity studies
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