Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-429-0 | CAS number: 95-53-4
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
Description of key information
With a BCF of < 1.3 at 100 µg/L and < 13 at 10 µg/L the substance has a very low potential for bioaccumulation.
Key value for chemical safety assessment
- BCF (aquatic species):
- 13 dimensionless
Additional information
As there is no data available for o-toluidine, read across with p-toluidine (CAS No. 106-49-0) is proposed to cover this endpoint. o-Toluidine and p-toluidine are isomers and have very similar chemical structures. Their main physico-chemical parameters (vapour pressure, water solubility, octanol/water partition coefficient, organic carbon partition coefficient and dissociation constant) are in the same range. The main ecotoxicological parameters like short-term toxicity on Daphnia and fish, toxicity on algae, and long-term toxicity on Daphnia are also in the same range. It is shown that Daphnia is the most sensitive aquatic organism for both isomers. Therefore a read across between these two substances to cover physico-chemical and ecotoxicological endpoints for o-toluidine seems to be justified. More details regarding the read across justification are depicted in the annex of the Chemical Safety Report (CSR).
According to a guideline which corresponds to OECD Guideline 305C (Bioaccumulation: Test for the degree of bioconcentration in Fish), a BCF of < 1.3 at 100 µg/L and < 13 at 10 µg/L was determined on fish (Cyprinus carpio) for p-toluidine. The test was conducted by EA, Japan: "Investigation of the Ecotoxicological Effects of OECD High Production Volume Chemicals", Office of Health Studies, Environmental Health Department, Environment Agency, Japan.
Additionally experimental bioconcentration factors (BCF) for o-toluidine indicate no significant potential for bioaccumulation in Pacific oysters (Crassostrea gigas) or Common bay mussels (Mytilus edulis). Oysters and mussels were exposed to 5 mg/L of o-toluidine in seawater for 24 hours, at 15 °C and pH 7.9. o-Toluidine carbon was 14C-ring-labelled, 3 replicates were conducted.
The steady state of the body burden was reached after 2 - 4 hours of exposure for the oysters and 4 hours for the mussels.
The obtained BCF values were 4.2 and 4.6 for mussels and oyster, respectively.
Supplemental the Kow value of 1.4 for o-toluidine indicates a low potential for bioaccumulation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
