4,4'-methylenebis[2-chloroaniline]

Dermal absorption

Administrative data

Endpoint:

dermal absorption in vitro / ex vivo

Type of information:

other: publication

Adequacy of study:

supporting study

Study period:

1982

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The data concern a literature study; human neonatal foreskin is isolated and placed in a diffusing chamber. The integrity of the cell layers is only checked histologically; the experiment contains no sufficient positive control on integrity of the cell layer.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1983

Report date:

1982

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

Freshly human neonatal foreskin was prepared to form a layer between two compartments. *MOCA was added to the upper compartment, samples were taken at t = 1, 2, 3, and 4 hours.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Radiolabelling:

yes

Remarks:

14C

Test animals

Species:

human

Strain:

other: neonatal foreskin

Administration / exposure

Duration of exposure:

t = 1, 2, 3, and 4 hr.

Details on in vitro test system (if applicable):

SKIN PREPARATION
- Source of skin: neonatal foreskin
- Membrane integrity check: solely histologically

Applicant's summary and conclusion

Conclusions:

The authors suggest that *MOCA is rapidly absorbed through the human foreskin. Since, the integrity of the cell layer is not sufficiently controlled, the data may be considered as doubtful.