Anisole

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

No data

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Study performed on 1946, no details were available but conducted according to accepted scientific methods.

Data source

Reference

Reference Type:

publication

Title:

The Toxicity and Toxic Manifestations of 2,2-Bis-(p-Chlorophenyl)-1,1,1-Trichloroethane (DDT) as Influenced by Chemical Changes in the Molecule. A Contribution to the Relation Between Chemical Constitution and Toxicological Action.

Author:

Oettingen WF and Sharpless NE

Year:

1946

Bibliographic source:

Journal of Pharmacology and Experimental Therapeutics 88:400-413

Materials and methods

Test guideline

Qualifier:

no guideline available

Principles of method if other than guideline:

No data

GLP compliance:

no

Remarks:

(before GLP statement)

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Anisole

EC Number:

202-876-1

EC Name:

Anisole

Cas Number:

100-66-3

Molecular formula:

C7H8O

IUPAC Name:

anisole

Test material form:

not specified

Details on test material:

- Name of test material (as cited in study report): Anisole
No other data was available.

Test animals

Species:

mouse

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation:20-30 grams.
- Housing: Mice were housed individually in wire cages.
- Diet (e.g. ad libitum): Fed Purina dog chow with each dose.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

The calculated doses were introduced into the stomach through the esophagus by means of a tuberculin syringe with a blunt needle.

Doses:

5, 10, and 25% solutions in olive oil.

No. of animals per sex per dose:

10 males per dose

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: 1 week
- Other examinations performed: Mice were observed for tremors, convulsions, and fatalities.

Statistics:

No data

Results and discussion

Preliminary study:

No data

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

2 800 mg/kg bw

Based on:

test mat.

Mortality:

four of eight animals died at a dose of 2800 mg/kg.

Clinical signs:

other: Only moderate depression was caused with lower doses while higher doses primary excitement then progressive depression, rapid and labored respiration and finally coma and death.

Gross pathology:

No data

Other findings:

Animals showed marked cyanosis in the final stages of poisoning.

Any other information on results incl. tables

None

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

The oral LD50 of Anisole to male white mice by gavage was determined to be 2800 mg/kg bw. Based on this study, Anisole is not classified according to EU GHS criteria and is classified Acute. Tox. Cat. 5, H303 according to UN GHS criteria.

Executive summary:

The acute oral toxicity of Anisole was determined in mice. The test was administered to 10 males per dose by gavage route at 5, 10, and 25% solutions in olive oil. The animals were observed over a 1-week period for tremors, convulsions, and fatalities. No further details were provided.

Four of eight animals died at a dose of 2800 mg/kg. Only moderate depression was caused with lower doses while higher doses primary excitement then progressive depression, rapid and labored respiration and finally coma and death.

The oral LD50 of Anisole to male white mice by gavage was determined to be 2800 mg/kg bw.

Based on these results, Anisole is not classified for acute toxicity according to the Regulation (EC) 1272/2008 (CLP).