Anisole

Basic toxicokinetics

Administrative data

Endpoint:

basic toxicokinetics in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study well described

Data source

Reference

Reference Type:

publication

Title:

Effets de l'anisole, de l'anéthole, du butylhydroxyanisole et du safrole sur le métabolisme de la flore caecale isolée, chez le rat

Author:

Fritsch P, de Saint-Blanquat G, Derache R, Canal T

Year:

1975

Bibliographic source:

European Journal of Toxicology, mai-juin 1975, 8, n°3, p. 169-174

Materials and methods

Objective of study:

metabolism

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The aim of this in vitro study was to assess the impact of 4 methoxybenzene substances including Anisole on the metabolism of the cecal flora in rats. Cecal flora was isolated from Wistar male rats and was incubated up to 60 minutes (10, 20, 30, 40, 50 and 60 minutes) with different concentrations of methoxybenzene substances (100, 200 and 400 µg/ml). A control without KOH was done and the effects of tween substances were examined.

GLP compliance:

no

Remarks:

before GLP statement

Test material

Test material information

Constituent 1

Reference substance name:

Anisole

EC Number:

202-876-1

EC Name:

Anisole

Cas Number:

100-66-3

Molecular formula:

C7H8O

IUPAC Name:

anisole

Test material form:

not specified

Details on test material:

no data

Radiolabelling:

no

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: ca. 280 g
- Fasting period before study: 17h
No more data.

Administration / exposure

Route of administration:

other: test on cecal flora directly

Vehicle:

other: Tween 20 at 1%

Details on exposure:

To isolate the cecal flora, wistar rats had a fasting period of 17 h, were sacrified by decapitation, at the same time of the day.
The cecal content was rapidly sampled and placed in 10 mL Krebs Ringer Phosphate (KRP), Ph 7.4 and 4°C.
A first centrifugation at 500 rpm during 2 mn eliminated food debris.
A second centrifugation at 4000 rpm during 10 mn (cold temperature), permitted the sedimentation of bacteria cells which were diluted in 5 mL of KRP, pH 7.4 and 4°C. After suspension of this media, aliquots were sampled in order to do incubation and dosage of DNA.
Metabolism study was realised with Warburg apparatus. All incubations were realised in strictly anaerobic conditions, with azote gas exempted of oxygen. The bacteria suspension was placed in buffered media containing 2% glucose.
The test items included Anisole were added at doses of 100, 200 and 400 µg/mL. Anisole was dispersed in Tween 20 at 1%.
Incubations were followed up to 60 mn and gaseous emission was reported to DNA of cecal bacteria, dosed according to the method of Dishes modified by Burton.
Controls were bacteria cells alone.

Duration and frequency of treatment / exposure:

Not applicable

Doses / concentrations

Remarks:

Doses / Concentrations:
100, 200 and 400 µg/mL

No. of animals per sex per dose / concentration:

No data

Control animals:

other: bacteria cells coming from rat cecal flore

Details on dosing and sampling:

PHARMACOKINETIC STUDY (Absorption, distribution, excretion)
- Tissues and body fluids sampled (delete / add / specify): cecal flora
- Time and frequency of sampling: 1 time, just after sacrifice (fasting period of 17h)
- From how many animals: (samples pooled or not): no data

Statistics:

Statistical comparison between controls and doses used were done by the couple test.
If necessary, regression curves were calculated (gaseous emission/time).

Results and discussion

Any other information on results incl. tables

Anisole was practically without effect on bacteria cecal flore coming from rats.

Validity of the experimental conditions was verified.

The couple test was employed, for each measured time.

The obtained value in the comparison system was never significant with Anisole.

Applicant's summary and conclusion

Conclusions:

Anisole had no effect on the caecal flora in rats.

Executive summary:

In this study (1975), the authors assessed the effects of four methoxybenzene food additives (anisole, anethole, butylhydroxyanisole, safrole) on the metabolism of isolated cecal flora coming from rats (in vitro test).

Anisole had practically no effect on cecal bacteria activities at concentrations close to the dietary levels. In the contrary safrole, and especially butylhydroxyanisole, inhibited bacterial metabolism at the same doses. Anethol had similar effects compared to anisole.