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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

One study (Cokgor et al, 2007) was selected as key study for endpoint coverage and is the only study carried forward for PNEC-STP derivation. The study determined a 30 min EC50 of 33 mg Ni/L.

Key value for chemical safety assessment

EC50 for microorganisms:
33 mg/L

Additional information

Only a few internationally accepted test methods for microorganisms exist, such as the OECD N° 209 (inhibition of respiration of activated sludge) and ISO N° 9509 (inhibition of nitrification). Generally, short-term measurements (in terms of hours) are preferred, generally corresponding with typical retention times in biological STPs. The TGD (EC, 2003) suggests 10 h as a preferable test duration. Furthermore, the information available has to be relevant for the processes that are potentially at risk of disruption, e. g. microbial degradation activity in an STP. To assess risks to these processes, microbial endpoints such as respiration and nitrification inhibition are considered to be the most relevant. Testing using a mixed microbial inoculum is considered more relevant than using single-species inoculum. Thus information reported on individual bacterial species like Microtox (with Vibrio fisherias test organism), Pseudomonas putida, Pseudomonas fluorescens and even Escherichia coli are therefore considered as less relevant than those from mixed inoculum.

Studies assessing the effects of nickel on ciliated protozoa (preferably T. pyriformis) and respiration/nitrification using bacteria originating from sewage treatment plants were regarded as directly relevant for the derivation of a PNEC STP. The key publication selected for Ni-PNEC STP derivation is Cokgor et al (2007). No other PNEC relevant studies that investigated the effects of Ni on bacterial populations were identified. However, the other studies in the database not deemed directly relevant, supported the relevancy and the conservative nature of an EC50of 33 mg/L.


CONCLUSION EC50 33 mg/L Ni (=  EC50 54 mg/L NiF2)