Pentapotassium triphosphate

Long-term toxicity to fish

Administrative data

Endpoint:

fish early-life stage toxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

Basic data given: comparable to guideline but missing analytical data

Data source

Reference

Reference Type:

publication

Title:

Effect of sodium tripolyphosphate (STPP) on the embryonic developmental stages of Zebrafish Brachydanio (danio) rerio

Author:

Sinha P and Kanamadi RD

Year:

2000

Bibliographic source:

Environment and ecology 18(4): 1007-1010

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)

Deviations:

yes

Remarks:

No data on dissolved oxygen or test substance concentration over time are given.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Pentasodium triphosphate

EC Number:

231-838-7

EC Name:

Pentasodium triphosphate

Cas Number:

7758-29-4

Molecular formula:

Na5P3O10 H5-xP3O10Nax (where x is approximately 5) 6H2O.Na5P3O10

IUPAC Name:

Pentasodium triphosphate

Details on test material:

- Name of test material (as cited in study report): Sodium tripolyphosphate

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Source: Local commercial supplier


METHOD FOR PREPARATION AND COLLECTION OF FERTILIZED EGGS
- Numbers of parental fish (i.e. of females used to provide required number of eggs): 20
- Method of collection of fertilised eggs: Not stated

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

None

Test conditions

Hardness:

110 mg/l (as CaCO3)

Test temperature:

26-27°C

pH:

7.4

Dissolved oxygen:

6.9 mg/l

Details on test conditions:

TEST SYSTEM
- No. of organisms per vessel:10


OTHER TEST CONDITIONS
- Photoperiod:14hL/14hD

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

5 mg/L

Basis for effect:

mortality

Details on results:

- Mortality/survival at embryo and larval stages: 4%
- Other biological observations: Abnormal pigmentation in some. Early hatch

Any other information on results incl. tables

Teratogenic effects of different concentrations of sodium tripolyphosphate on Brachydanio embryos

Concentration mg/l	No of embryos exposed	% mortality at 96h	% abnormality at 96h	Abnormality
Control	50	0	0	-
5	50	4	24	Abnormal pigmentation. Some early hatch
10	50	12	48	Many embryos showed abrupt cell proliferations during cleavage. Less pigmentation and absence of it in some cases
20	50	58	100
40	50	86	100	Retarded growth, early hatch, abnormal body structure in some

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The LOEC of sodium tripolyphosphate was found to be 5 mg/L.