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Short-term toxicity to fish

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Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1984
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: The duration of the test (48h) is too short for an acute fish toxicity test.
Qualifier:
according to
Guideline:
other: DIN 38 412 Teil Part 15 (1982) and Method of the German Standards Institution, Berlin, Germany
GLP compliance:
no
Analytical monitoring:
no
Test organisms (species):
other: Leuciscus idus L.
Details on test organisms:
TEST ORGANISM
- Common name: Golden Orfe
- Strain: Leuciscus idus L.
- Age at study initiation (mean and range, SD):
- Length at study initiation (length definition, mean, range and SD): 5 - 8 cm
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Test temperature:
20 + 1 C
pH:
6.3 - 7.5
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 10 L
- Aeration: Yes
- No. of organisms per vessel: 10
- Biomass loading rate: 10 animals per concentration


Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
2 300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
> 2 300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC100
Effect conc.:
3 300 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Conclusions:
The 48 hour LC100 was 3300 mg /L
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
Cited as Directive 92/69/EEC, C.1
GLP compliance:
yes
Analytical monitoring:
yes
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: Zebra Fish
- Strain: Danio rerio
- Source: AquaKloger
- Length at study initiation (length definition, mean, range and SD): 2.5 - 3.5 cm
- Weight at study initiation (mean and range, SD): Not mentioned


Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Hardness:
13.9 °dH (248.1 mg/l CaCO3)
Test temperature:
21.6 - 22.2 C
pH:
7.6 - 7.8
Dissolved oxygen:
8.0 - 8.5 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:glass aquarium, 5 l test media
- Aeration: Gentle
- No. of organisms per vessel: 10
- Biomass loading rate: 1.12 g body weight (wet weight) per liter


TEST MEDIUM / WATER PARAMETERS
Total Hardness: 13.9 °dH (248.1 mg/l CaCO3)

OTHER TEST CONDITIONS
- Adjustment of pH: 7.6 - 7.8
- Photoperiod:16 hours light, 8 hours dark

Reference substance (positive control):
no
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
>= 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality


No effects were observed during the study.

Conclusions:
The measured concentration of >= 100 mg/l correspond to 91 % of nominal value at 96 hours. Mortalities during acclimatisation period > 5 %
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: 1a GLP guideline study
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
OECD TG no. 203 (2004)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
The same day as every 24-hour exposure period, 50 g of Polyol R3530 was dissovled per one litre of aerated dilution water as s stocksolution.

Composition of dilution water:
1. Calcium chloride: Dissolve 11.76 g CaCl2 x 2H2O in water and dilute to 1 litre.
2. Magnesium sulphate: Dissolve 4.93 g MgSO4 x 7H2O in water and dilute to 1 litre.
3. Sodium bicarbonate: Dissolve 2.59 g NaHCO3 in water and dilute to 1 litre.
4. Potassium chloride: Dissolve 0.23 g KCl in water and dilute to 1 litre.
Mix 1 - 4 in water and dilute to 40 litres. Before use the water should be oxygenated with an air pump, a plastic tube with sintered end. Teh pH value may be adjusted with HCl or NaOH. Adjusted water should not be aerated again. The dilution water should allow survival of zebra fish for at least 96 hrs. Reverse osmosis (RO) water was used to prepare the dilution water.

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
250 +/- 25 mg CaCo3 / L
Test temperature:
24 +/- 1 °C
pH:
7.2 to 7.9
Dissolved oxygen:
> 90 % (use of aeration)
Salinity:
no data
Nominal and measured concentrations:
Nominal concentrations: 3200, 1600, 800, 400 and 200 mg/L for exposure test, and 0 mg/L for control test
Measured concentrations: no data
Details on test conditions:
light : dark rythm = 16 : 8
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
550 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
200 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
Effect concentrations are given to be based on measured values. However, there is no data on the measured concentration and if effect concentration is based on initially measured concentration or concentration that was measured after 24 hours of exposure (both measurements were carried out).

Visible abnormalities (e.g. darker pigmentation, loss of equilibrium, disturbance in swimming behaviour or increased respiration rate) were observed on everyone fish in concentration 400 mg/L as nominal concentration.

Mortality in the test and control group during the 96 hours of exposure:

Group Concentration
mg/L
Time (hours)
0 3 24 48 72 96 Total
Control 0 0 0 0 0 0 0 0
Test group 200 0 0 0 0 0 0 0
Test group 400 0 0 0 0 0 1 1
Test group 800 0 0 0 7 7 7 7
Test group 1600 0 0 6 7 7 7 7
Test group 3200 0 7 7 7 7 7 7

Validity criteria fulfilled:
yes
Conclusions:
Short-term toxicity was determined according to OECD method no. 203 and given as 96 hour-LC50 = 550 mg/L. Furthermore, the 96 hour-NOEC is given as 200 mg/L (Perstorp, 2010).
Executive summary:

Short-term toxicity was determined according to OECD method no. 203 and given as 96 hour-LC50 = 550 mg/L. Furthermore, the 96 hour-NOEC is given as 200 mg/L (Perstorp, 2010).

Description of key information

The 96 hr LC50 were reported as > 100 mg/l (EU Method C1 , Danio rerio).

Key value for chemical safety assessment

Additional information

Acute toxicity to fish has been investigated with two freshwater species: Two studies with Danio rerio (96 hr) and one study with Leuciscus idus (48hr). The 96 hr LC50 were reported as >100 mg/l. The measured concentration of >= 100 mg/l correspond to 91 % of nominal value at 96 hours. Mortalities during acclimatisation period > 5 %