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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
other: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Study period:
28 Jun - 27 Aug 2010
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 105 (Water Solubility)
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method A.6 (Water Solubility)
Deviations:
no
GLP compliance:
yes (incl. QA statement)
Remarks:
Food and Consumer Product Safety Authority, Prinses Beatrixlaan 2, 2595 AL Den Haag, Postbus 19506, 2500 CM Den Haag, The Netherlands, 30 March 2009
Type of method:
flask method
Water solubility:
38.7 g/L
Temp.:
20 °C
pH:
> 3.5 - < 3.6
Remarks on result:
other: At a loading rate of 100 g/L the water solubility of the test substance was 38.7 g/L. It corresponds with a water solubility percentage compared to the loading rate of 39%.
Water solubility:
268 g/L
Temp.:
20 °C
pH:
3.5
Remarks on result:
other: At the maximum loading rate of 1:1 w/v (maximum loading rate according to the guidelines) the water solubility of the test substance was 268 g/L. It corresponds with a water solubility of 27% compared to the loading rate.
Details on results:
Vinasses is a UVCB product. The flask method at two loading rates was chosen for the determination of the water solubility of the test substance.

The water solubility of the test substance at 20 °C increased with the loading rate of the samples.

Preliminary test                

The content of the test substance in the water sample at the loading rate of 100 g/L and 1000 g/L (1:1 w/v ratio) was 41.4 g/L and 283 g/L, respectively.

 

The preliminary test demonstrated that the test substance contains a large fraction of components with a relatively high water solubility. According to this, the maximum loading rate of 1000 g/L (1:1 w/v) and a 10-times lower loading rate of 100 g/L were applied in the main study.

 

Main study                

The results for the samples at the loading rates of 100 g/L and 1:1 w/v taken at 24, 48 and 72 hours are given in the tables below.

 

The maximum difference (MD) of the 24-hour sample at the loading rate of 100 g/L was somewhat lower than the 48- and 72- hour measurements (maximum difference > 15%). According to the guidelines, the water solubility of the test substance was given as the mean value of the 48- and 72-hour measurements.

 

At a ratio of 1:1 w/v, the MD of the concentrations determined in the 24, 48 and 72 hour test samples was < 15%. According to the guidelines, the water solubility of the test substance is given as the mean value of the analysed concentrations.

 

The TOC background of the blank water sample was maximum 1.3% compared to the lowest content of the test samples. It was considered not significantly affect the results of the test samples.

 

Main study - water solubility at a loading rate of 100 g/L

Loading rate

[g/L]

Stirring time

[hours]

Analysed concentration

[g/L]

Mean


[g/L]

Percentage to
loading rate
[%]

MD

[%]

pH

 

 

 

 

 

 

 

100

24

31.4

 

 

 

3.5

 

 

 

 

 

 

 

100

48

37.6

 38.71

 391

 5.71

3.5

 

 

 

 

 

 

 

100

72

39.8

 

 

 

3.6

 

 

 

 

 

 

 

1              Obtained with the samples at 48 and 72 hours.

 

Main study - water solubility at a loading rate of 1:1 w/v

Loading rate

[g/L]

Stirring time

[hours]

Analysed concentration

[g/L]

Mean


[g/L]

Percentage to
loading rate
[%]

MD

[%]

pH

 

 

 

 

 

 

 

1000

24

260

268

27

4.8

3.5

 

 

 

 

 

 

 

1000

48

273

 

 

 

3.5

 

 

 

 

 

 

 

1000

72

273

 

 

 

3.5

 

 

 

 

 

 

 

Conclusions:
Interpretation of results (migrated information): other: The test substance contains a large fraction of components with a relatively high water solubility . However, the test substance was not completely soluble in water since in all samples undissolved test substance particles were observed.
Vinasses is a UVCB product. The flask method at two loading rates was chosen for the determination of the water solubility of the test substance.

The water solubility of the test substance at 20 °C increased with the loading rate of the samples. At a loading rate of 100 g/L the water solubility of the test substance was 38.7 g/L. It corresponds with a water solubility percentage compared to the loading rate of 39%. The pH of the aqueous samples was 3.5 – 3.6.

At the maximum loading rate of 1:1 w/v (maximum loading rate according to the guidelines) the water solubility of the test substance was 268 g/L. It corresponds with a water solubility of 27% compared to the loading rate. The pH of the samples was 3.5.

Based on the results obtained it was concluded that the test substance contains a large fraction of components with a relatively high water solubility (i.e. 268 g/L at a 1:1 w/v loading rate). The test substance was not completely soluble in water since in all samples undissolved test substance particles were observed.

Description of key information

At a loading rate of 100 g dry mass/L :
38.7 g/L at 20 °C (corresponds to a water solubility percentage of 39% of the dry substance).

At the maximum loading rate of 1000 g dry mass/L :
268 g/L at 20 °C (corresponds to a water solubility percentage of 27% of the dry substance).

Not completely soluble in water since in all samples undissolved test substance particles were observed.

Key value for chemical safety assessment

Additional information

Water solubility is derived from a test with Vinasses, residue of fermentation containing biomass of Corynebacterium glutamicum according to OECD Guideline 105, flask method.