Amines, C12-18-alkyldimethyl, N-oxides

Long-term toxicity to aquatic invertebrates

Description of key information

One long term (21 days) toxicity to Daphnia magna study is available for C12-14 AO. No effect concentration of 0.7 mg AO/L was reported in the study.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

0.7 mg/L

Additional information

The long-term toxicity of the substance was evaluated in a 21 day survival and reproduction test with Daphnia magna, following the OECD 211 guideline [Maki AW (1977)]. Four replicates per concentration, 5 organisms per replicate, were tested in a flow-through system. Mean measured test concentrations were: 0, 0.08, 0.17, 0.34, 0.70, and 1.35 mg AO/L.

Survival was monitored at 24 hours, 96 hours, 7 days, and daily thereafter. Reproduction was monitored beginning with the production of the first brood on day 7 or 8, and daily thereafter. LC50s and EC50s were calculated by probit analysis. Results are based on the mean measured concentration of the amine oxide. The 21 -day NOEC was 0.70 mg AO/L, based on both survival and reproduction. The 21 day LC50 was 0.96 mg AO/L. The 21-day EC50’s, based on total young production, average brood size, and % days reproduction occurred, were 0.88 mg AO/L, 1.01 mg AO/L, and 1.04 mg AO/L, respectively.

Based on the large overlap in chain length distributions between the two substances, similarities in physico-chemical properties and acute toxicity to Daphnia, it is considered that this result can be read across to C12 -18 AO.